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Antimicrobial surfaces based on self-assembled nanoreactors : from block copolymer synthesis to bacterial adhesion study
| Content Provider | Semantic Scholar |
|---|---|
| Author | Cottenye, Nicolas |
| Copyright Year | 2010 |
| Abstract | S. .....................................................................................................................................................................................5 ENGLISH............................................................................................................................................................................... 5 FRANÇAIS. ........................................................................................................................................................................... 6 ACKNOWLEDGMENT. ..................................................................................................................................................................7 CONTENT. .........................................................................................................................................................................................8 GENERAL INTRODUCTION. .................................................................................................................................................... 12 BIOFILMS AND THEIR CONTROL. .......................................................................................................................................12 AMPHIPHILES, VESICLES, AND THEIR APPLICATIONS. ......................................................................................................15 SCOPE OF THE THESIS. .......................................................................................................................................................17 REFERENCES. .....................................................................................................................................................................19 PART I: POLYMERS, NANOREACTORS, AND HYBRIDIZATION FOR CONTROLLED SURFACE PREPARATION.............................................................................................................................................................................. 22 A. Introduction. ..................................................................................................................................................................... 23 B. State of the art. .................................................................................................................................................................. 25 B.1 Amphiphilic block copolymers, amphiphilic ―chimeras‖ their properties and synthesis. ..............................................25 B.2 Nanoreactors: Definition, description and limitations. ................................................................................................28 B.3 Prodrug-drug system. ................................................................................................................................................31 B.4 Immobilization of vesicles to surfaces: possible strategies. .........................................................................................33 B.5 Oligonucleotide hybridization. ..................................................................................................................................35 B.6 Vesicles mechanical properties. .................................................................................................................................36 C. Materials and methods. .................................................................................................................................................... 37 C.1 Oligonucleotide-based amphiphilic block copolymer synthesis. ..................................................................................37 C.1.a Materials. ....................................................................................................................................................... 37 C.1.b Solid phase synthesis. ..................................................................................................................................... 38 C.2 Chemical analysis of the diblock copolymer. .............................................................................................................38 C.2.a Nuclear magnetic resonance (NMR). ............................................................................................................... 38 C.2.b Fourier Transform Infrared Spectroscopy (FTIR). ............................................................................................ 39 C.2.c Elementary analysis. ....................................................................................................................................... 39 C.3 UV-Vis spectroscopy for polymer concentration quantification. .................................................................................39 C.4 Self-assembly of the diblock copolymer. ...................................................................................................................39 C.5 Self-assembly characterisation and chain configuration. .............................................................................................39 Antimicrobial surface based on self-assembled nanoreactors: from block copolymer synthesis to bacterial adhesion study 9 2010 Nicolas Cottenye C.5.a Circular Dichroism Spectrocscopy (CD). ......................................................................................................... 39 C.5.b Transmission Electron Microscopy (TEM). ..................................................................................................... 40 C.5.c Scanning Electron Microscopy (SEM). ............................................................................................................ 40 C.5.d Dynamic Light Scattering (DLS). .................................................................................................................... 40 C.6 Nanoreactor preparation and characterization. ............................................................................................................40 C.6.a Materials. ....................................................................................................................................................... 40 C.6.b Nanoreactor preparation. ................................................................................................................................. 41 C.6.c Nanoreactor purification. ................................................................................................................................ 41 C.6.d Other samples and sample names. ................................................................................................................... 42 C.6.e Polymer and enzyme concentration determination. ........................................................................................... 43 C.6.f Enzyme activity monitoring and concentration determination from Michaelis-Menten kinetics measurement by fluorescence spectroscopy. ....................................................................................................................................... 44 C.7 Vesicle Labeling. ......................................................................................................................................................45 C.8 Oligonucleotide modified surfaces.............................................................................................................................45 C.8.a Materials. ....................................................................................................................................................... 45 C.8.b Oligonucleotide self-assembled monolayer (SAM) modified surfaces............................................................... 45 (i) Protocol for ―low density‖ oligonucleotide modified surfaces. ...................................................................45 (ii) Protocol for―high density‖oligonucleotide modified surfaces. ...................................................................46 C.8.c Type of oligonucleotide modified surfaces....................................................................................................... 46 C.9 Vesicle modified surfaces..........................................................................................................................................47 C.9.a Surfaces on the immobilization of vesicles through nonspecific base pairing..................................................... 47 C.9.b Surfaces resulting from the immobilization of vesicles though hybridization. ................................................... 47 C.9.c Reference surfaces. ......................................................................................................................................... 47 C.10 Surface characterization. .........................................................................................................................................48 C.10.a Fluorescence microscopy. ............................................................................................................................. 48 C.10.b X-ray Photoelectron Spectroscopy (XPS). ..................................................................................................... 48 C.10.c Contact angle measurements. ........................................................................................................................ 48 C.10.d Ellipsometry. ................................................................................................................................................ 49 C.10.e Atomic force microscopy (AFM). .................................................................................................................. 49 C.10.f Quartz crystal microbalance with dissipation monitoring QCM-D. .................................................................. 49 CHAPTER 1. BLOC COPOLYMER SYNTHESIS AND CHARACTERIZATION..... |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://tel.archives-ouvertes.fr/docs/00/59/85/60/PDF/2010MULH5351_these_Cottenye.pdf |
| Alternate Webpage(s) | https://edoc.unibas.ch/1264/1/Cottenye_PhD_thesis_2010.pdf |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Atomic-force microscopy Bacterial Adhesion Base Pairing Circular dichroism Copolymer Crossbreeding Crystal Arthropathies DLS format Fluorescence Spectroscopy Forty Nine Immobiliser Infrared Rays Kinesiology Magnetic Resonance Imaging Microscopy, Atomic Force Name Nuclear Magnetic Resonance Photoelectric effect Photon Correlation Spectroscopy Polymer Prodrugs Purification of quantum state Quantitation Quartz Scanning Electron Microscopy Self-assembled monolayer Self-assembly Spectroscopy, Fourier Transform Infrared Transmission Electron Microscopy Trial Elements Domain Ultraviolet Photoelectron Spectroscopy Vesicle (morphologic abnormality) X-Ray Computed Tomography X-Ray Photoelectron Spectroscopy |
| Content Type | Text |
| Resource Type | Article |
