Loading...
Please wait, while we are loading the content...
Similar Documents
Maintaining Eastern newts (Notophthalmus viridescens) for regeneration research.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Simon, Hans-Georg Odelberg, Shannon |
| Copyright Year | 2015 |
| Abstract | The adult Eastern newt, Notophthalmus viridescens, has long served as a model for appendage as well as heart muscle regeneration studies. Newt tissues include all major cell types known in other vertebrates and mammals, including bone, cartilage, tendon, muscle, nerves, dermis, and epidermis. Therefore, these aquatic salamanders make an excellent model for studying the regeneration of complex tissues. Regeneration of adult tissues requires the integration of new tissues with preexisting tissues to form a functioning unit through a process that is not yet well understood. Scale is also an issue, because the regenerating tissues or structures are magnitudes larger than their embryonic counterparts during development, and therefore, it is likely that different physics and mechanics apply. Regardless, regeneration recapitulates to some degree developmental processes. In this chapter, we will describe basic methods for maintaining adult Eastern newts in the laboratory for the study of regeneration. To determine similarities and differences between development and regeneration at the cellular and molecular level, there is also a need for embryonic newt tissue. We therefore also outline a relatively simple way to produce and raise newt embryos in the laboratory. |
| Starting Page | 17 |
| Ending Page | 25 |
| Page Count | 9 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.springer.com/cda/content/document/cda_downloaddocument/9781493924943-c1.pdf?SGWID=0-0-45-1494786-p177229488 |
| Alternate Webpage(s) | http://www.springer.com/cda/content/document/cda_downloaddocument/9781493924943-c1.pdf?SGWID=0-0-45-1494786-p177229488 |
| PubMed reference number | 25740474v1 |
| Alternate Webpage(s) | https://doi.org/10.1007/978-1-4939-2495-0_2 |
| DOI | 10.1007/978-1-4939-2495-0_2 |
| Journal | Methods in molecular biology |
| Volume Number | 1290 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Body tissue Bone Tissue Cartilage Dermis Embryo Epidermis Large Mechanics Muscle Myocardium Natural regeneration Nerve Notophthalmus Tbox1 protein, Newt Tendon structure appendage |
| Content Type | Text |
| Resource Type | Article |
