Low mitochondrial DNA and ATP contents contribute to the absence of birefringent spindle imaged with PolScope in in vitro matured human oocytes.

Content Provider	Semantic Scholar
Author	Zeng, Hai-Tao Yeung, William Shu-Biu Shu, Yi-Min Xu, Yan-Wen Zhuang, Guang-Lun Liang, Xiaoyan
Copyright Year	2007
Abstract	BACKGROUND Birefrigent meiotic spindle in live human oocytes can be visualized by the PolScope. This study investigated the relationship between birefrigent meiotic spindle and cytoplasmic mitochondrial DNA (mtDNA) and ATP contents in in vitro matured human oocytes. METHODS Oocytes at germinal vesicle stage were collected and cultured for 24-48 h with or without the metabolic inhibitor, carbonyl cyanide p-(tri-fluromethoxy) phenyl-hydrazone (FCCP). All in vitro matured oocytes were examined by PolScope for the presence of meiotic spindle, then the oocytes were used for either intracytoplasmic sperm injection or the measurement of mitochondrial quantity and ATP content. RESULTS Meiotic spindles were observed in 51.3% (60/117) of the in vitro matured oocytes. Oocytes with detectable meiotic spindle contained significantly higher mtDNA copies (637 250 +/- 237 606 versus 491 454 +/- 153 406, P = 0.027) and ATP content (1.97 +/- 0.38 versus 1.65 +/- 0.32 pmol, P = 0.028) when compared with those without detectable meiotic spindle. However, in vitro matured oocytes showed a significantly reduced rate of positive meiotic spindle and a lower ATP content when cultured with FCCP. A lower incidence of normal fertilization and good quality embryos were observed if meiotic spindles were not detected. CONCLUSIONS Low mtDNA and ATP content might contribute to the absence of birefringent spindle imaged with the PolScope in human in vitro matured oocytes.
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PubMed reference number	17449512v1
Volume Number	22
Issue Number	6
Journal	Human reproduction
Language	English
Access Restriction	Open
Subject Keyword	Adenosine Triphosphate Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone Contain (action) Copy (object) Cyanides DNA, Mitochondrial Embryo Fertilization Hydralazine Hydrazones Meiotic Spindle Apparatus Metabolic Process, Cellular Mitochondrial Inheritance Sperm Injections, Intracytoplasmic Spermatozoa Cell Count Vesicle (morphologic abnormality) contents - HtmlLinkType germinal vesicle picomole (pM)
Content Type	Text
Resource Type	Article