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1 Crystal structure of archaeal chromatin protein Alba 2-dsDNA complex from Aeropyrum pernix K 1
| Content Provider | Semantic Scholar |
|---|---|
| Author | Tanaka, Tomoyuki N. Padavattan, Sivaraman Kumarevel, Thirumananseri |
| Copyright Year | 2012 |
| Abstract | All thermophilic and hyperthermophilic archaea encode homologs of dimeric Alba (Sac10b) proteins that bind cooperatively at high density to DNA. Here, we report the 2.0 Å resolution crystal structure of an Alba2 (Ape10b2)-dsDNA complex from Aeropyrum pernix K1. A rectangular tube-like structure encompassing duplex DNA reveales the positively charged residues in the monomer-monomer interface of each dimer packing on either side of the bound dsDNA in successive minor grooves. The extended hairpin loop connecting the strands β3-β4 undergoes significant conformational changes upon DNA-binding to accommodate the other Alba2-dimer during oligomerization. Mutational analysis of key interacting residues confirmed the specificity of Alba2-dsDNA interactions. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.jbc.org/content/early/2012/02/10/jbc.M112.343210.full.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |