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Rac regulates collagen-induced HSP27 phosphorylation via p44/p42 MAP kinase in human platelets.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Kageyama, Yasunari Doi, Tomoaki Akamatsu, Shigeru Kuroyanagi, Gen Kondo, Akira Mizutani, Jun Otsuka, Takanobu Tokuda, Haruhiko Kozawa, Osamu Ogura, Shinji |
| Copyright Year | 2013 |
| Abstract | We previously reported that the collagen-induced phosphorylation of heat shock protein (HSP) 27 via p44/p42 mitogen-activated protein (MAP) kinase is sufficient to induce the secretion of platelet-derived growth factor (PDGF)-AB and the release of soluble CD40 ligand (sCD40L) from human platelets. It has been shown that Rac, which belongs to the Rho family of small GTPases, is involved in the collagen-induced platelet aggregation. In this study, we investigated the role of Rac in the collagen-stimulated release of PDGF-AB and sCD40L in human platelets. Human blood was donated from healthy volunteers and platelet-rich plasma was obtained from the blood samples. The samples were then treated with 1.0 µg/ml collagen for 0, 1, 3, or 5 min and Rac1 activity was determined using the Rac1 Activation Assay kit. We found that collagen stimulated the activation of Rac in human platelets in a time-dependent manner. However, pre-treatment with NSC23766, a selective inhibitor of Rac-guanine nucleotide exchange factor interaction, reduced the collagen-induced platelet aggregation. NSC23766 markedly attenuated not only the collagen-induced p44/p42 MAP kinase phosphorylation, but also the phosphorylation of HSP27 at three serine residues (Ser-15, Ser-78 and Ser-82). In addition, the collagen‑induced release of PDGF-AB and sCD40L was significantly suppressed by NSC23766 in a dose-dependent manner. These results strongly suggest that Rac regulates the collagen-induced HSP27 phosphorylation via p44/p42 MAP kinase in human platelets, resulting in the stimulation of PDGF-AB secretion and the release of sCD40L. |
| Starting Page | 130 |
| Ending Page | 130 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.spandidos-publications.com/ijmm/32/4/813/download |
| PubMed reference number | 23900529v1 |
| Alternate Webpage(s) | https://doi.org/10.3892/ijmm.2013.1455 |
| DOI | 10.3892/ijmm.2013.1455 |
| Journal | International journal of molecular medicine |
| Volume Number | 32 |
| Issue Number | 4 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Blood Platelet Disorders Blood Platelets Blood specimen Guanine Nucleotide Exchange Factors Guanine Nucleotides Guanosine Triphosphate Phosphohydrolases Heat shock proteins Heat-Shock Response Ligands Mast/Stem Cell Growth Factor Receptor Kit, human Mitogens NSC 23766 Platelet Count measurement RAC-Alpha Serine/Threonine Kinase eIF-2 Kinase |
| Content Type | Text |
| Resource Type | Article |
