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A New Chinese Isolate of Strawberry Mild Yellow Edge Virus from Fragaria Pentaphylla
| Content Provider | Semantic Scholar |
|---|---|
| Author | Yang |
| Copyright Year | 2011 |
| Abstract | Strawberry mild yellow edge virus (SMYEV), an aphidtransmitted potexvirus, is one of the most economically important viral pathogens of strawberries worldwide. SMYEV was first reported from China more than 20 years ago (Wang, 1988). In surveys conducted from 2005 to 2009, 53 out of 93 samples collected from different Chinese regions proved to be SMYEV-positive when tested by RT-PCR using the specific primer set [SMYEVF(+): 5’CCG CTG CAG TTG TAG GGT A-3’ and SMYEVR():5’-CAT GGC ACT CAT TGG AGC TGG G-3’] (Lamprecht and Jelkmann, 1997). Some samples were also indexed on the sensitive indicator clones EMC, UC5, and UC10. A wild strawberry (F. pentaphylla) from Gansu, was also positive by RT-PCR and biological indexing. A 930 bp sequence (GenBank accession No. EU107084) of the 3’ terminal region of the SMYEV genome of the isolate from F. pentaphylla was obtained by 3’ rapid amplification of cDNA ends (RACE). Comparisons revealed that this sequence shared 79.5 to 86.6% identity at the nucleotide level with published SMYEV sequences. Phylogenetic analysis showed that all Chinese SMYEV isolates fell into four clades which, to some extent, were related with the geographical origin of the isolates. However, the viral isolate from F. pentaphylla was comprised in a distinct clade. To our knowledge, this is the first characterization of a Chinese isolate of SMYEV from a wild strawberry source. Additional and more extensive surveys should be carried out to ascertain whether additional and more diversified SMYEV strains occur in China. |
| Starting Page | 4 |
| Ending Page | 76 |
| Page Count | 73 |
| File Format | PDF HTM / HTML |
| Volume Number | 93 |
| Alternate Webpage(s) | http://www.sipav.org/main/jpp/index.php/jpp/article/download/2387/1064 |
| Alternate Webpage(s) | https://doi.org/10.4454/jpp.v93i4.2386 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
