NDLI: ICA512/IA-2抗体新規RIA報の臨床的評価 - Radioligand binding assay法との比較検討 -

Please wait, while we are loading the content...

ICA512/IA-2抗体新規RIA報の臨床的評価 - Radioligand binding assay法との比較検討 -

Content Provider	Semantic Scholar
Author	英二, 川崎康徳, 世羅成裕, 藤田幸弘, 安部三爵子, 山内方子, 尾崎治子, 宮副賢一, 山川茂雄, 魚谷博文, 瀧野浩則, 山崎義彦, 山口勝美, 江口
Copyright Year	2000
Abstract	Clinical Evaluation of New Radioimmunoassay for ICA 512/IA-2 Autoantibodies Comparative Analysis with Radioligand BindingAssayEiji Kawasaki, Yasunori Sera, Naruhiro Fujita, Takahiro Abe, Mikako Yamauchi, Masako Ozaki, Haruko Miyazoe, Kenichi Yamakawa, Shigeo Uotani, Hirofumi Takino, Hironori Yamasaki, Yoshihiko Yamaguchi and Katsumi Eguchi The First Department of Internal Medicine, Nagasaki University School of Medicine, Nagasaki, Japan To determine the assay accuracy of a newly developed radioimmunoassay (RIA) kit for ICA 512/IA-2 autoantibodies, we analyzed kit disease sensitivity and specificity compared to highly sensitive radioligand binding assay (REA) using sera from 72 patients with type 1 diabetes, 127 patients with type 2 diabetes, and 71 healthy subjects. The prevalence of ICA 512/IA-2 autoantibodies by RIA and REA were 43.1% and 48.6% in type 1 diabetics, and 3.9% and 2.4% in type 2 diabetics. No healthy subjects were positive in either assay (specificity 100%). Although ICA 512/IA-2 autoantibody levels correlated significantly between RIA and REA, some sera showed negative results in RIA but positive in REA in a low titer range. With analysis using the standard deviation score (SDS) in each serum from patients with type 1 diabetes, we observed some sera with high SDS by REA but low SDS by RIA even though all were positive. The newly developed RIA has high specificity but lower disease sensitivity than REA. Re-evaluation of the sera using the assay with higher sensitivity or combined analysis with other islet-autoantibodies required in type 1 diabetic patients negative for ICA 512/IA-2 autoantibodies measured by this RIA. J.JapanDiab.Soc.43 (12) : 1051-1056,2000
Starting Page	1051
Ending Page	1056
Page Count	6
File Format	PDF HTM / HTML
Volume Number	43
Alternate Webpage(s)	http://naosite.lb.nagasaki-u.ac.jp/dspace/bitstream/10069/22601/1/tonyobyo43_1051.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in