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Sri Lanka CYTOLOGICAL EXAMINATION OF MICROSPORE DEVELOPMENT FOR MICROSPORE AND ANTHER CULTURE OF COCONUT ( Cocos nucifera L . ) cv SRI LANKA
| Content Provider | Semantic Scholar |
|---|---|
| Author | Tall Perera, P. I. P. |
| Copyright Year | 2008 |
| Abstract | Production of double haploids through anther and microspore culture has a considerable potential for shortening the breeding cycle in coconut. The developmental stage of a microspore is a critical factor in androgensis and microspores at late uninucleate stage have been shown to be the most suitable stage for in vitro culture for many crop species. Routine cytological ; examination of microspores for identifying the correct stage for culture is tedious and time-consuming. In the present study, a correlation between the microspore developmental stage and age of the spadix (in Sri Lanka Tall coconut) in terms of weeks-before-splitting (WBS) was established for practical convenience of anther and microspore culture. At the age of eight WBS, anthers consisted of microspore mother cells. Within one week, these cells underwent meiosis and produced tetrads. The release of microspores from tetrads could be observed in six WBS spadices. Microspores at late uninucleate stage, which is reported as the most suitable stage for androgenesis, could be obtained from three WBS spadices. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://cocos.sljol.info/articles/10.4038/cocos.v15i0.2188/galley/1829/download/ |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
