NDLI: Real-time reverse transcriptase PCR assay for detection of human metapneumoviruses from all known genetic lineages.

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Real-time reverse transcriptase PCR assay for detection of human metapneumoviruses from all known genetic lineages.

Content Provider	Semantic Scholar
Author	Maertzdorf, Jeroen Wang, Chiaoyin K. Brown, Jennifer B. Quinto, Joseph D. Chu, Marla Graaf, Miranda De Hoogen, Bernadette G. Van Den Spaete, Richard R. Osterhaus, Albert D. M. E. Fouchier, Ron A. M.
Copyright Year	2004
Abstract	The discovery of human metapneumovirus and its implications for respiratory tract disease have emphasized the need for a sensitive, specific, and rapid assay to detect this virus in a clinical setting. It recently became clear that human metapneumovirus can be grouped into at least four genetic lineages. Previously described assays for the detection of human metapneumovirus were developed by using limited sequence information and failed to detect viruses from all four genetic lineages with comparable sensitivities. Here we describe the development and evaluation of a real-time reverse transcriptase PCR assay that detects human metapneumovirus from the four known genetic lineages with equal specificities and sensitivities.
Starting Page	71S
Ending Page	76S
Page Count	6
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://repub.eur.nl/pub/10320/15004041.pdf
Alternate Webpage(s)	http://citeseerx.ist.psu.edu/viewdoc/download;jsessionid=099CD2D7A9AB8A78D0B9D7F165B08587?doi=10.1.1.123.9739&rep=rep1&type=pdf
Alternate Webpage(s)	http://jcm.asm.org/content/42/3/981.full.pdf
PubMed reference number	15004041v1
Volume Number	42
Issue Number	3
Journal	Journal of clinical microbiology
Language	English
Access Restriction	Open
Subject Keyword	HIV reverse transcriptase gene mutations detected:Prid:Pt:Isolate:Nom Metapneumovirus Respiratory System Respiratory Tract Diseases Reverse Transcriptase Polymerase Chain Reaction Virus
Content Type	Text
Resource Type	Article

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