NDLI: Dysregulation of MicroRNA-196b-5p and MicroRNA-375 in Gastric Cancer

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Dysregulation of MicroRNA-196b-5p and MicroRNA-375 in Gastric Cancer

Content Provider	Semantic Scholar
Author	Lee, Seung Woo Park, Ki Cheol Kim, Jeong Goo Moon, Sung Jin Kang, Sang Bum Lee, Dong Soo Sul, Hae Joung Ji, Jeong Seon Jeong, Hyun-Yong
Copyright Year	2016
Abstract	PURPOSE Dysregulated microRNAs (miRNAs) can contribute to cancer development by leading to abnormal proliferation of cells, apoptosis, and differentiation. Although several miRNAs that are related to gastric cancer have been identified, the reported results have been inconsistent. The aim of this study was to determine miRNA expression profiles and validate miRNAs up- and down-regulated in gastric cancer. MATERIALS AND METHODS We evaluated 34 primary gastric cancer tissues and paired adjacent nontumorous gastric tissues. Total RNA was extracted, and low-molecular-weight RNAs (<200 nucleotides) were isolated for further analysis. Two pairs of tissues were processed for GeneChip microarray analysis, and the identified up- and down-regulated miRNAs were validated by real-time quantitative polymerase chain reaction (qPCR). RESULTS In the set of differentially expressed miRNAs, 5 were overexpressed by more than 2 fold, and 5 were reduced by 2 fold or less in gastric cancer tissues compared with normal gastric tissues. Four of these miRNAs (miR-196b-5p, miR-375, miR-483-5p, and miR-486-5p) were then validated by qPCR, and the relative expression levels of 2 miRNAs (miR-196b-5p and miR-375) were significantly different between cancer and normal tissues. CONCLUSIONS Our results revealed that the expression of miR-196b-5p and miR-375 significantly correlates with gastric cancer. These miRNAs could therefore serve as diagnostic biomarkers of gastric cancer.
Starting Page	221
Ending Page	229
Page Count	9
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://pdfs.semanticscholar.org/82ee/7b8cb92086b224f561afe0cc5e527542db16.pdf
PubMed reference number	5206312
Alternate Webpage(s)	https://doi.org/10.5230/jgc.2016.16.4.221
DOI	10.5230/jgc.2016.16.4.221
Journal	Journal of gastric cancer
Volume Number	16
Language	English
Access Restriction	Open
Subject Keyword	Apoptosis Biological Markers Body tissue Extraction Greater Than Malignant neoplasm of stomach Maxillary right first premolar prosthesis MicroRNAs Microarray Analysis Neoplasms Nucleotides Polymerase Chain Reaction RNA Stage IV Ovarian Carcinoma Stomach Carcinoma
Content Type	Text
Resource Type	Article

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