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Kolot Core-binding speci ® city of bacteriophage integrases
| Content Provider | Semantic Scholar |
|---|---|
| Abstract | The site-speci®c recombination systems of bacteriophages k and HK022 share the same mechanism and their integrase proteins show strong homology. Nevertheless the integrase protein of each phage can only catalyze recombination between its own att sites. Previous work has shown that the speci®city determinants in the att sites are located within the sequences that bind the integrase to the core of att. DNA fragments that carry attL and attR sites of each phage were challenged with each of the two integrases and the DNA-protein complexes were examined by the gelretardation technique. The results show that each integrase can form higher-order DNA-protein complexes only with its cognate att sites, suggesting that dierences in the mode of binding to the core are responsible for the speci®city dierence between the two integrases. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://page-one.springer.com/pdf/preview/10.1007/s004380051209 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
