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Atherosclerotic plaque smooth muscle cells have a distinct phenotype.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Mulvihill, Eileen R. Jaeger, Jochen Sengupta, Rimli Ruzzo, Walter L. Reimer, Cecile Lukito, Sheila Schwartz, Stephen M. |
| Copyright Year | 2004 |
| Abstract | OBJECTIVE The present study addresses the question, "Are plaque smooth muscles cells (SMCs) genetically distinct from medial SMCs as reflected by the ability to maintain a distinctive expression phenotype in vitro?" METHODS AND RESULTS Multiple cell strains were developed from carotid endarcterectomy specimens, and quadruplicate array hybridizations were completed for each sample. A new normalization protocol was developed and used to analyze the data. Permutation analysis suggests that most of the significant differences in expression could not have occurred by chance. A broad pattern of significant expression differences, consisting of almost 5% of the genes probed, was detected. Quantitative polymerase chain reaction (QPCR) confirmation was found in 70% of a subset of genes selected for validation. CONCLUSIONS The SMC cultures were nearly indistinguishable by morphological features, population doubling time, and sensitivity to cell death induced by Fas cross-linking. Surprisingly, array expression analysis identified differences so extensive that we conclude that plaque and medial SMCs are distinctly different SMC cell types. |
| Starting Page | 286 |
| Ending Page | 286 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://atvb.ahajournals.org/content/atvbaha/24/7/1283.full.pdf |
| Alternate Webpage(s) | http://atvb.ahajournals.org/content/atvbaha/24/7/1283.full.pdf?download=true |
| PubMed reference number | 15142862v1 |
| Volume Number | 24 |
| Issue Number | 7 |
| Journal | Arteriosclerosis, thrombosis, and vascular biology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Atherosclerosis Cell Death Cessation of life Dental Plaque Real-Time Polymerase Chain Reaction Senile Plaques Single-Chain Antibodies Smooth muscle (tissue) Specimen Subgroup cell type confirmation - ResponseLevel |
| Content Type | Text |
| Resource Type | Article |
