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Universidade Federal Rural De Pernambuco Pró-reitoria De Pesquisa E Pós-graduação Programa De Pós-graduação Em Biociência Animal
| Content Provider | Semantic Scholar |
|---|---|
| Author | Ferri, Rinaldo Cavalcante |
| Copyright Year | 2016 |
| Abstract | This study aimed to investigate the presence of Toxoplasma gondii and Neospora caninum in naturally infected sheep and goats, employing immunological, molecular and histopathological techniques. In the first experiment, sera and tissues of 100 slaughtered sheep were used in the state of Alagoas. For the detection of antibodies to T. gondii the immunofluorescent antibody test (IFAT) was used and for the detection of parasites in tissue techniques of Polymerase Chain Reaction (PCR) and Immunohistochemistry (IHC) were used only in positive animals in serology. To study the agreement between the direct techniques we employed the Kappa test. IFAT, 14% (14 /100) were positive animals with no significant difference for males and females. In PCR, 21.43 % (03/14) and IHC positive staining was observed for T. gondii in brain tissue of 7.14 % (1 /14) of sheep. Histopathologically, the predominant finding was the mononuclear cell infiltrate in the heart, brain and cerebellum around blood vessels, followed by hemorrhage in the medulla and cerebellum. The agreement between the direct diagnosis techniques in sheep naturally infected was moderate (K = 0.44), so it is important to involve a more direct diagnostic technique in the diagnosis of ovine toxoplasmosis. In the second experiment, sera and tissues of 100 slaughtered sheep and tissues of 10 fetuses were used. For the detection of antibodies to N. caninum IFAT technique was used and the detection of parasites in the tissues PCR and IHC were used only in positive serology in animals.To study the agreement between the direct techniques we employed the Kappa test. IFAT, 13 % (13/100) of the animals were positive, where 7.7% (1/13) were male and 92.3 % (12/13) females, predominantly under 800, with a statistically significant difference for between males and females. PCR of adult sheep and fetuses, 38.5 % and 30 % showed a positive reaction, respectively, mainly in the brain. In IHC, two adult sheep showed positive staining in the brain. Histopathologic findings revealed mononuclear infiltrates in the heart, brain and spinal cord and areas of congestion in the brain, foci of hemorrhage and mononuclear infiltrates in the brain and spinal cord and vessels of the brain and cerebellum. N. caninum is present in sheep slaughtered in the state of Alagoas and vertical transmission was observed in the natural way. The agreement between the direct diagnosis techniques in sheep naturally infected was moderate (K = 0.44), so it is important to involve a more direct diagnostic technique in the diagnosis of infection by this parasite. In the third experiment were collected a total of 78 samples (brain, liver, lung, kidney and heart) of 17 aborted fetuses from goats and sheep on 10 farms in the State of Pernambuco, Brazil. Samples were analyzed by PCR, histopathology and immunohistochemistry for T. gondii and N. caninum. No sample was positive for T. gondii by PCR and IHC. In PCR to N. caninum eleven samples (14.1%) were positive in a sample of brain, five of liver, one of lung, two of kidneys and two of heart. Histopathology in the samples that showed no autolysis were evidenced mainly mild mononuclear infiltration in the liver and foci of congestion in the regions juxtaglomerular and medullar of the kidney. IHC on a sample of goat fetal brain showed positive reaction to N. caninum. The results confirm the vertical transmission of N. caninum in naturally infected sheep and goats in northeastern Brazil. Key-words: sheep, diagnosis, Toxoplasma gondii, Neospora caninum |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://tede2.ufrpe.br:8080/tede/bitstream/tede2/4477/2/Adrianne%20Mota%20de%20Alcantara.pdf |
| Alternate Webpage(s) | http://www.pgba.ufrpe.br/sites/ww4.pgba.ufrpe.br/files/documentos/dissertacao_breno_bezerra_aragao.pdf |
| Alternate Webpage(s) | http://www.pgba.ufrpe.br/sites/ww4.pgba.ufrpe.br/files/documentos/diagnostico_imunologico_molecular_e_histopatologico_das_infeccoes_por_toxoplasma_gondii_e_neospora_caninum_em_caprinos_e1.pdf |
| Alternate Webpage(s) | http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/4580/2/Flaviana%20Santos%20Wanderley.pdf |
| Alternate Webpage(s) | http://www.pgba.ufrpe.br/sites/ww4.pgba.ufrpe.br/files/documentos/dissertacao_mestrado_adrianne_-_versao_final.pdf |
| Alternate Webpage(s) | http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/7199/2/Breno%20Bezerra%20Aragao.pdf |
| Alternate Webpage(s) | http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/7870/2/Eulina%20Tereza%20Nery%20Farias.pdf |
| Alternate Webpage(s) | http://www.pgba.ufrpe.br/sites/ww4.pgba.ufrpe.br/files/documentos/tese_rinaldo_ferri_biociencia.pdf |
| Alternate Webpage(s) | http://www.tede2.ufrpe.br:8080/tede2/bitstream/tede2/4563/2/Rinaldo%20Cavalcante%20Ferri.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
