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Intracoronary Administration of Allogeneic Adipose Tissue–Derived Mesenchymal Stem Cells Improves Myocardial Perfusion But Not Left Ventricle Function, in a Translational Model of Acute Myocardial Infarction
| Content Provider | Semantic Scholar |
|---|---|
| Author | Bobí, Joaquim Solanes, Núria Jiménez, Rodrigo Fernández‐ Arriola, Carlos Galán‐ Dantas, Ana Paula Villela Friera, Leticia Fernández‐ Gálvez-Montón, Carolina Monzó, Elisabet Rigol‐ Agüero, Jaume Ramírez, José Roqué, Mercé Bayes-Genis, Antoni González, Javier Sánchez‐ García-Álvarez, Ana Cristina Sabaté, Manel Roura, Santiago Ibanez, Borja Rigol, Montserrat Negre |
| Copyright Year | 2017 |
| Abstract | BACKGROUND Autologous adipose tissue-derived mesenchymal stem cells (ATMSCs) therapy is a promising strategy to improve post-myocardial infarction outcomes. In a porcine model of acute myocardial infarction, we studied the long-term effects and the mechanisms involved in allogeneic ATMSCs administration on myocardial performance. METHODS AND RESULTS Thirty-eight pigs underwent 50 minutes of coronary occlusion; the study was completed in 33 pigs. After reperfusion, allogeneic ATMSCs or culture medium (vehicle) were intracoronarily administered. Follow-ups were performed at short (2 days after acute myocardial infarction vehicle-treated, n=10; ATMSCs-treated, n=9) or long term (60 days after acute myocardial infarction vehicle-treated, n=7; ATMSCs-treated, n=7). At short term, infarcted myocardium analysis showed reduced apoptosis in the ATMSCs-treated animals (48.6±6% versus 55.9±5.7% in vehicle; P=0.017); enhancement of the reparative process with up-regulated vascular endothelial growth factor, granulocyte macrophage colony-stimulating factor, and stromal-derived factor-1α gene expression; and increased M2 macrophages (67.2±10% versus 54.7±10.2% in vehicle; P=0.016). In long-term groups, increase in myocardial perfusion at the anterior infarct border was observed both on day-7 and day-60 cardiac magnetic resonance studies in ATMSCs-treated animals, compared to vehicle (87.9±28.7 versus 57.4±17.7 mL/min per gram at 7 days; P=0.034 and 99±22.6 versus 43.3±14.7 22.6 mL/min per gram at 60 days; P=0.0001, respectively). At day 60, higher vascular density was detected at the border zone in the ATMSCs-treated animals (118±18 versus 92.4±24.3 vessels/mm2 in vehicle; P=0.045). Cardiac magnetic resonance-measured left ventricular ejection fraction of left ventricular volumes was not different between groups at any time point. CONCLUSIONS In this porcine acute myocardial infarction model, allogeneic ATMSCs-based therapy was associated with increased cardioprotective and reparative mechanisms and with better cardiac magnetic resonance-measured perfusion. No effect on left ventricular volumes or ejection fraction was observed. |
| File Format | PDF HTM / HTML |
| DOI | 10.1161/JAHA.117.005771 |
| PubMed reference number | 28468789 |
| Journal | Medline |
| Volume Number | 6 |
| Alternate Webpage(s) | https://repisalud.isciii.es/bitstream/20.500.12105/5119/1/IntracoronaryAdministrationOfAllogeneic_2017 |
| Alternate Webpage(s) | http://jaha.ahajournals.org/content/ahaoa/6/5/e005771.full.pdf?download=true |
| Alternate Webpage(s) | http://diposit.ub.edu/dspace/bitstream/2445/124319/1/681518.pdf |
| Journal | Journal of the American Heart Association |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
