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Spatial and dynamic changes in intracellular Ca2+ measured by confocal laser-scanning microscopy in bullfrog sympathetic ganglion cells
| Content Provider | Semantic Scholar |
|---|---|
| Author | Kuba, Kenji Hua, Shao-Ying Nohmi, Mitsuo |
| Copyright Year | 1991 |
| Abstract | Confocal laser scanning microscopy (CLSM) was used to record spatial and dynamic changes in the intracellular Ca2+ [(Ca2+]i) of bullfrog sympathetic ganglion cells in excised tissue or in culture. A CLSM utilizing Ar ion laser (488 nm) and recording fluo-3 fluorescence yielded the sliced image of ganglion cells, while conventional epifluorescence microscopy provided the cell image of a convex structure. A high K+ (50 mM) solution, caffeine (3-10 mM) and electrical stimulation (10-20 Hz, 0.5-10 s) caused a homogeneous increase in fluo-3 fluorescence with or without regional differences, possibly due to intracellular organelles and other constituents. Scanning a single horizontal line across the cytoplasm with He-Cd laser (325 nm) and recording indo-1 fluorescence demonstrated that the rate of rise in [Ca2+]i following action potentials depends on the distance from the cell membrane and on the cytoplasmic constituents, showing an inward spread of 'Ca(2+)-wave' at variable speeds of 17-219 microns/s. These results suggest that heterogeneity of the cytoplasmic structures and constituents affects dynamic and spatial changes of [Ca2+]i in response to stimuli in neurones. Such heterogenic changes in [Ca2+]i would better be studied by CLSM. |
| Starting Page | 245 |
| Ending Page | 259 |
| Page Count | 15 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/0168-0102(91)90082-A |
| PubMed reference number | 1652720 |
| Journal | Medline |
| Volume Number | 10 |
| Alternate Webpage(s) | https://api.elsevier.com/content/article/pii/016801029190082A |
| Alternate Webpage(s) | https://www.sciencedirect.com/science/article/pii/016801029190082A?dgcid=api_sd_search-api-endpoint |
| Alternate Webpage(s) | https://doi.org/10.1016/0168-0102%2891%2990082-A |
| Journal | Neuroscience Research |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
