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Escherichia coli Rho factor is involved in lysis of bacteriophage T4-infected cells.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Linder, C. H. Carlson, Katrin |
| Copyright Year | 1985 |
| Abstract | A Rid (Rho interaction deficient) phenotype of bacteriophage T4 mutants was defined by cold-sensitive restriction (lack of plaque formation) on rho+ hosts carrying additional polar mutations in unrelated genes, coupled to suppression (plaque formation) in otherwise isogenic strains carrying either a polarity-suppressing rho or a multicopy plasmid expressing the rho+ allele. This suggests that the restriction may be due to lower levels of Rho than what is available to T4 in the suppressing strains.--Rid394 X 4 was isolated upon hydroxylamine mutagenesis and mapped in the t gene; other t mutants (and mot, as well as dda dexA double mutants) also showed a Rid phenotype. In liquid culture in strains that restricted plaque formation Rid394 X 4 showed strong lysis inhibition (a known t- phenotype) but no prolonged phage production (another well-known t- phenotype). This implies that when Rho is limiting the t mutant shuts off phage production at the normal time. Lysis inhibition was partially relieved, and phage production prolonged to varying extents depending on growth conditions in strains that allowed plaque formation. No significant effect on early gene expression were found. Apparently, both mutant (polarity-suppressing) and wild-type Rho can function in prolonging phage production and partially relieving lysis inhibition of Rid394 X 4 when present at a sufficiently high level, and Rho may play other role(s) in T4 development than in early gene regulation. |
| Starting Page | 197 |
| Ending Page | 218 |
| Page Count | 22 |
| File Format | PDF HTM / HTML |
| PubMed reference number | 3902562 |
| Journal | Medline |
| Volume Number | 111 |
| Issue Number | 2 |
| Alternate Webpage(s) | http://www.genetics.org/content/genetics/111/2/197.full.pdf |
| Journal | Genetics |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
