Benzo[a]pyrene up-regulates cyclooxygenase-2 gene expression in oral epithelial cells.

Content Provider	Semantic Scholar
Author	Kelley, Daniel J. Mestre, Juan R. Subbaramaiah, Kotha Sacks, Peter G. Schantz, Stimson Pryor Tanabe, Tadashi Inoue, Hiroyasu Ramonetti, John T. Dannenberg, Andrew J.
Copyright Year	1997
Abstract	Cyclooxygenase may be important in the pathogenesis of smoking-related cancer because it activates carcinogens and catalyzes prostaglandin biosynthesis. We determined the effects of benzo[a]pyrene (B[a]P), a polycyclic aromatic hydrocarbon in tobacco smoke, on cyclooxygenase-2 (Cox-2) mRNA, protein and synthesis of prostaglandin E2 (PGE2) in normal and transformed oral epithelial cells. Treatment with B[a]P caused a dose-dependent increase in production of PGE2, with a maximal increase of approximately 100%. Enhanced synthesis of PGE2 was associated with increased amounts of Cox-2 protein. B[a]P also caused a two-fold increase in Cox-2 mRNA in both normal and transformed cells. Transient transfections with a Cox-2 promoter construct showed that B[a]P-mediated induction of Cox-2 mRNA reflected increased transcription. Levels of Cox-1 were unaffected by B[a]P. B[e]P did not affect the synthesis of PGE2 or amounts of Cox-2. These data are important because B[a]P-mediated induction of Cox-2 may predispose to carcinogenesis by enhancing the production of mutagens and the synthesis of prostaglandins.
Starting Page	146807
Ending Page	146807
Page Count	1
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PubMed reference number	9111217v1
Volume Number	18
Issue Number	4
Journal	Carcinogenesis
Language	English
Access Restriction	Open
Subject Keyword	AROMATICS Anabolism Benzo(a)pyrene Carcinogenesis Gene Expression Hydrocarbons Mutagens Neoplasms PTGS1 protein, human Polycyclic Hydrocarbons, Aromatic Prostaglandin E2 Prostaglandin-Endoperoxide Synthase Prostaglandins Tobacco smoke Transfection cyclooxygenase 2 prostaglandin E synthase
Content Type	Text
Resource Type	Article