NDLI: Bluetongue virus induces apoptosis in cultured mammalian cells by both caspase-dependent extrinsic and intrinsic apoptotic pathways

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Bluetongue virus induces apoptosis in cultured mammalian cells by both caspase-dependent extrinsic and intrinsic apoptotic pathways

Content Provider	Semantic Scholar
Author	Nagaleekar, Viswas Konasagara Tiwari, Ashok K. Kataria, Ranjit S. Bais, Manish V. Ravindra, P. V. Kumar, Singh Umesh
Copyright Year	2007
Abstract	SummaryBluetongue virus (BTV) causes haemorrhagic disease in sheep and induces death in cultured mammalian cells. In the present study, BTV-induced apoptotic pathways in Vero cells were elucidated. Cells infected with BTV at 0.1 m.o.i underwent DNA fragmentation and membrane blebbing within 48 h postinfection. BTV-induced apoptosis was blocked by the pan-caspase inhibitor, z-VAD-FMK. Immuno-blotting using anti-caspase-8 and -9 antibodies detected the activation of the respective caspases. Flow cytometry analyses following 3, 3′ dihexyloxacarbocyanine iodide staining revealed the loss of mitochondrial membrane potential. Our study confirms the involvement of both caspase-dependent extrinsic and intrinsic pathways of apoptosis in BTV-infected cells.
Starting Page	1751
Ending Page	1756
Page Count	6
File Format	PDF HTM / HTML
DOI	10.1007/s00705-007-0988-z
Alternate Webpage(s)	https://page-one.springer.com/pdf/preview/10.1007/s00705-007-0988-z
PubMed reference number	17530353
Alternate Webpage(s)	https://doi.org/10.1007/s00705-007-0988-z
Journal	Medline
Volume Number	152
Journal	Archives of Virology
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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