NDLI: Fractionation of carbon (13C/12C) isotopes in glycine decarboxylase reaction.

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Fractionation of carbon (13C/12C) isotopes in glycine decarboxylase reaction.

Content Provider	Semantic Scholar
Author	Ivlev, Alexander A. Bykova, Natalia V. Igamberdiev, Abir U.
Copyright Year	1996
Abstract	Fractionation of carbon isotopes (13C/12C) by glycine decarboxylase (GDC) was investigated in mitochondrial preparations isolated from photosynthetic tissues of different plants (Pisum, Medicago, Triticum, Hordeum, Spinacia, Brassica, Wolffia). 20 mM glycine was supplied to mitochondria, and the CO2 formed was absorbed and analyzed for isotopic content. CO2 evolved by mitochondria of Pisum was enriched up to 8% in 12C compared to the carboxylic atom of glycine. CO2 evolved by mitochondria of the other plants investigated was enriched by 5-16% in 13C. Carbon isotope effects were sensitive to reaction conditions (pH and the presence of GDC cofactors). Theoretical treatment of the reaction mechanism enabled us to conclude that the value and even the sign of the carbon isotope effect in glycine decarboxylation depend on the contribution of the enzyme-substrate binding step and of the decarboxylation step itself to the overall reaction rate. Therefore, the fractionation of carbon isotopes in GDC reaction was revealed which provides essential isotopic effects in plants in addition to the well-known effect of carbon isotope fractionation by the central photosynthetic enzyme, ribulose-1,5-biphosphate carboxylase.
File Format	PDF HTM / HTML
DOI	10.1016/0014-5793(96)00421-8
PubMed reference number	8647275
Journal	Medline
Volume Number	386
Issue Number	2-3
Alternate Webpage(s)	https://core.ac.uk/download/pdf/82498766.pdf
Alternate Webpage(s)	https://doi.org/10.1016/0014-5793%2896%2900421-8
Journal	FEBS letters
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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