Loading...
Please wait, while we are loading the content...
Similar Documents
Antitumoral effects of Melissa officinalis on breast cancer in vitro and in vivo.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Saraydin, Serpil Unver Tuncer, Ersin Tepe, Bektaş Karadayi, Şule Özer, Hatice Sen, M. Berke Karadayi, Kursat Inan, Deniz Elagöz, Şahande Polat, Zübeyde Akın Duman, Mustafa Turan, Mustafa |
| Copyright Year | 2012 |
| Abstract | BACKGROUND There is a long standing interest in the identification of medicinal plants and derived natural products for developing cancer therapeutics. Here we investigated the antiproliferative properties of Melissa officinalis (MO) from Turkey on breast cancer. METHODS MO extracts were studied for cytotoxicity against breast cancer cell lines (MCF-7, MDA-MB-468 and MDA-MB-231). In vitro apoptosis studies were performed by annexin V staining and flow cytometry analyses. Immunohistochemistry for Ki-67 and caspase 7 in the tumoral tissue sections of DMBA-induced mammary tumors in rats was also performed, along with TUNEL assays to detect apoptotic cells. In vivo anticancer activity testing was carried out with reference to inhibition of growth of DMBA induced mammary tumors in rats. RESULTS MO showed cytotoxicity against three cancer cell lines, inducing increase in Annexin-positive cells. Expression of caspase-7 protein and TUNEL positive cells were much higher in rats treated by MO, compared with the untreated control group, while expression of Ki-67 was decreased. Furthermore, in vivo studies showed that mean tumor volume inhibition ratio in MO treated group was 40% compared with the untreated rats. CONCLUSION These results indicated that MO extrcts have antitumoral potential against breast cancer. |
| Starting Page | 760 |
| Ending Page | 765 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://journal.waocp.org/article_26573_dcb2543ba88569f86a1e72d6fdcd8a05.pdf |
| Alternate Webpage(s) | http://ocean.kisti.re.kr/downfile/volume/apocp/POCPA9/2012/v13n6/POCPA9_2012_v13n6_2765.pdf |
| PubMed reference number | 22938456v1 |
| Volume Number | 13 |
| Issue Number | 6 |
| Journal | Asian Pacific journal of cancer prevention : APJCP |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | 3,4-Methylenedioxyamphetamine 9,10-Dimethyl-1,2-benzanthracene Animal Mammary Neoplasms Annexins Apoptosis Breast Cancer Cell Cultured Cell Line Flow Cytometry In Situ Nick-End Labeling MDA-MB-468 Melissa officinalis Natural Products Plants, Medicinal Staining method Tumor Volume caspase-7 |
| Content Type | Text |
| Resource Type | Article |
