NDLI: Development of Monoclonal Antibodies against HIV-1 p24 Protein and Its Application in Colloidal Gold Immunochromatographic Assay for HIV-1 Detection

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Development of Monoclonal Antibodies against HIV-1 p24 Protein and Its Application in Colloidal Gold Immunochromatographic Assay for HIV-1 Detection

Content Provider	Semantic Scholar
Author	Ni, Chao Dzakah, Emmanuel E. Wang, Haiying Kang, Keren Tang, Shixing Wang, Jihua Wang, Jufang
Copyright Year	2016
Abstract	Human immunodeficiency virus type 1 (HIV-1) p24 protein is the most abundant viral protein of HIV-1. This protein is secreted in blood serum at high levels during the early stages of HIV-1 infection, making it a biomarker for early diagnosis. In this study, a colloidal gold immunochromatographic assay (GICA) was established for detecting p24 protein using mouse monoclonal antibodies (mAbs). The HIV-1 p24 protein was expressed in E. coli strain BL21 and the purified protein was used to immunize mice. Stable hybridoma cell lines secreting anti-p24 monoclonal antibodies were obtained after ELISA screening and subcloning by limiting dilution. 34 different capture and labeling mAb pairs were selected by a novel antibody-capture indirect sandwich ELISA and then applied in GICA to detect p24 protein. The GICA method has a limit of detection (LOD) of 25 pg/mL and could detect p24 protein in all 10 positive samples obtained from the National Reference of HIV-1 p24 antigen. Out of 153 negative samples tested, 3 false positives results were obtained. The overall specificity of this test was 98.03%. The good sensitivity and specificity of this method make it a suitable alternative to provide a more convenient and efficient tool for early diagnosis of HIV infection.
File Format	PDF HTM / HTML
DOI	10.1155/2016/6743904
PubMed reference number	27069923
Journal	Medline
Volume Number	2016
Alternate Webpage(s)	http://downloads.hindawi.com/journals/bmri/2016/6743904.pdf
Alternate Webpage(s)	https://doi.org/10.1155/2016%2F6743904
Journal	BioMed research international
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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