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jmbReview Determination of Microbial Growth by Protein Assay in an Air-Cathode Single Chamber Microbial Fuel Cell
| Content Provider | Semantic Scholar |
|---|---|
| Author | Kakarla, Ramesh Moon, Jung Mi Min, Booki |
| Copyright Year | 2015 |
| Abstract | Wastewater treatment has been successfully carried out by conventional technologies such as activated sludge and trickling filters to remove organic compounds efficiently. However, the excess sludge production in wastewater treatment requires substantial energy input and efforts to treat and dispose of the sludge. About 25-65% of the total operational cost of a wastewater treatment plant can be accounted for sludge treatment [7]. In addition, energy costs for aeration will typically be 45-75% of all energy consumption in conventional bioreactor operation [4]. As a result, a novel technology needs to be developed to treat wastewater with less consumption of energy and sludge formation. Microbial fuel cells (MFCs) have been suggested as an innovative and environmentally friendly technology that can treat wastewater with simultaneous electricity generation [10]. Additionally, this MFC technology is considered to have low sludge production compared with an aerobic process. In general, the biomass yield under aerobic condition is around 0.4 to 0.64 g-COD-cell/g-COD-substrate, and in anaerobic condition, it is about 0.04 to 0.25 g-COD-cell/gCOD-substrate [3, 4, 8]. In MFC operation, the biomass yield was reported to range from 0.07 to 0.22 g-COD-cell/gCOD-glucose in the suspended solids [12]. However, the previous studies obtained the MFC biomass yield using a defined substrate such as acetate and glucose instead of real wastewater, and also direct comparative determination of the biomass yield between MFC and aerobic processes was not attempted. In this study, comparative biomass production was investigated using real domestic wastewater in an aircathode single chamber MFC and aerobic process. Protein concentration was determined using a protein assay kit for accurate calculation of active biomass [15]. The protein assay kit is considered as a more precise measurement of the total amount of bacteria, even at low concentration, compared with conventional volatile suspended solid (VSS) measurement where some cells are lost through a porous glass fiber filter [2]. Substrate degradation during microbial growth was obtained with soluble chemical oxygen demand (SCOD), and the biomass yield was calculated based on the protein and SCOD. The biomass Received: December 24, 2014 Revised: January 28, 2015 Accepted: February 3, 2015 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.jmb.or.kr/submission/Journal/025/JMB025-07-17_FDOC_1.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
