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Chiral Separation of a-Amino Acid Derivatives by Capillary Electrophoresis Using 6-Amino-6-deoxy-fi-cyclodextrin and Its N-Hexyl Derivative as Chiral Selectors
| Content Provider | Semantic Scholar |
|---|---|
| Author | Egashira, Naoyoshi Mutoh, Osamu Kurauchi, Yoshiaki Ohga, Kazuya |
| Copyright Year | 2006 |
| Abstract | Capillary electrophoresis (CE) is a promising analytical technique with the necessary high resolution in wideranging fields, including pharmacology and medical science. Efficient chiral separation is expected to be one of the most available abilities of CE; its utility can be even further extended when novel chiral selectors become more highly developed. Chiral separations have been effected by micellar electrokinetic capillary chromatography using chiral selectors, such as chiral surface-active agents having saccharide"2, amino acid3, and steroid moieties4'5, in some cases, in combination with cyclodextrins (CDs). CDs are also good additives in chiral separations by capillary zone electrophoresis (CZE); for example, CDs having methoxy6'', carboxyl8 and sulfonato groups9 have been used successfully. On the other hand, only one report has been presented, as far as we know, on the effect of a CD having a positively charged group on the chiral separation of amino acids: CDs having an amino group have shown a high chiral separation ability for 2-hydroxy acids because of their stable complex formations.10 In this paper we report on the chiral separations of N-(2,4dinitrophenyl)(DNP)and N-dansyl(DNS)-a-amino acids by CZE using 6-amino-6-deoxy-f3-cyclodextrin (ACD, Fig. l) and 6-deoxy-6-hexylamino-/3-cyclodextrin (HACD, Fig. 1) as chiral selectors. Tetraalkylammonium additives with short alkyl chains adsorbed on a capillary silica wall have improved chiral separation in CZE through controlling an electroosmotic flow.11 ACD having an amino group is also expected to adsorb on the capillary silica wall, and, thus, to produce a more effective chiral separation. Further, chiral separations with HACD have been compared to those with ACD on the basis of the hydrophobicity of the hexyl group on HACD. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.jstage.jst.go.jp/article/analsci1985/12/3/12_3_503/_pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
