Obtaining and characterization of monoclonal antibodies against capsular polysaccharides of Streptococcus pneumoniae serotype 1, 5, 6B, 14 and 19F

Content Provider	Semantic Scholar
Author	Bencomo, Fidel Ramírez Sánchez, Abel R. Fajardo Aznar, Elizabeth González Alfaro, Oscar Otero Arias, R. C. Martínez, Darcy Núñez Grogues, Reinaldo Acevedo
Copyright Year	2018
Abstract	Five monoclonal antibodies named 2B5B10, 2E4D11, 1D12E10, 1D1D11 and 177/10/27/11 were produced against capsular polysaccharides of Streptococcus pneumoniae serotypes 1, 5, 6B, 14 and 19F respectively. They all were obtained by hybridoma technology and produced by in vivo procedures. Were purified by Protein A-based Affinity Chromatography and the yield of all processes was around the 2.9 and 3.7 mg per mL of ascitic fluid; and with a high purity (over 90% in all cases). All MAbs only reacted against their homologue polysaccharide showing no cross reactivity against other serogroups. The affinity constants (Kaff) measured by non-competitive ELISA were 3.08 x 1011 M-1 for 2B5B10, 1.53 x 1012 M-1 for 2E4D11, 8.68 x 1010 M-1 for 1D12E10, 1.42 x 1013 M-1 for 1D1D11 and 7.2 109 M-1 for 177/10/27/11. The potential application of these MAbs for identity tests was demonstrated by their abilities of no cross-react with the other purified PS presents in QuimiVio, through indirect ELISA
File Format	PDF HTM / HTML
DOI	10.21931/rb/2018.03.01.4
Alternate Webpage(s)	http://www.revistabionatura.com/files/2018.03.01.4.pdf
Alternate Webpage(s)	https://www.revistabionatura.com/files/4-Vol-3-no-1-2018.pdf
Alternate Webpage(s)	https://doi.org/10.21931/rb%2F2018.03.01.4
Volume Number	3
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article