Crystal structure of calf spleen purine nucleoside phosphorylase complexed to a novel purine analogue

Content Provider	Semantic Scholar
Author	Pereiraa, Henrique M. G. Berdinib, V. Cleasbyb, A. Garratta, R. C.
Copyright Year	2007
Abstract	The combined use of a rapid virtual screen of a small fragment library together with a single point enzyme assay has been used for the discovery of novel PNP inhibitors. The availability of readily soakable crystals of bovine PNP has allowed the approach to be experimentally validated by determining the crystal structure of one of the inhibitor-PNP complexes. Comparison of the experimentally determined binding mode with that predicted by the virtual screening shows them to be similar. This represents a starting point for the growth of the ligand into a higher affinity inhibitor. 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://core.ac.uk/download/pdf/82574245.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article