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Enzymatic hydrolysis of pretreated sugar cane bagasse using Penicillium funiculosum and Trichoderma harzianum cellulases
| Content Provider | Semantic Scholar |
|---|---|
| Author | Nobuyuki, Roberto Serpa, Viviane Isabel Rocha, Vanessa Alves Lima Mesquita, Renata Aparecida Alves Anna, Lídia Maria Melo Santa Castro, Aline Machado De Driemeier, Carlos Eduardo Pereira, N. Polikarpov, Igor |
| Copyright Year | 2011 |
| Abstract | Abstract In this study, we investigated the enzymatic hydrolysis of pretreated sugarcane bagasse using eight different enzymatic blends obtained from concentrated crude enzyme extracts produced by Penicillium funiculosum and Trichoderma harzianum as well as from the extracts in combination with a commercial enzymatic cocktail. The influence of different levels of biomass delignification, degree of crystallinity of lignicellulose, composition of enzymatic activities and BSA on enzymatic hydrolysis yields (HYs) was evaluated. Our X-ray diffraction studies showed that crystallinity of lignocellulose is not a key determinant of its recalcitrance toward enzymatic hydrolysis. In fact, under the experimental conditions of our study, an increase in crystallinity of lignocellulosic samples resulted in increased glucose release by enzymatic hydrolysis. Furthermore, under the same conditions, the addition of BSA had no significant effect on enzymatic hydrolysis. The most efficient enzyme blends were obtained by mixing a commercial enzymatic cocktail with P. funiculosum or T. harzianum cellulase preparations (HYs above 97%) followed by the concentrated extract of P. funiculosum alone (HY = 88.5%). Increased hydrolytic efficiencies appeared to correlate with having an adequate level of both β-glucosidase and xylanase activities in the blends. |
| Starting Page | 1196 |
| Ending Page | 1201 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/j.procbio.2011.01.022 |
| Volume Number | 46 |
| Alternate Webpage(s) | https://core.ac.uk/download/pdf/82436147.pdf |
| Alternate Webpage(s) | https://doi.org/10.1016/j.procbio.2011.01.022 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
