Loading...
Please wait, while we are loading the content...
Similar Documents
Linkage between catecholate siderophores and the multicopper oxidase CueO in Escherichia coli.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Grass, Gregor Thakali, Keshari M. Klebba, Phillip E. Thieme, Daniel Müller, Axel R. Von Wildner, Günter F. Rensing, Christopher |
| Copyright Year | 2004 |
| Abstract | The multicopper oxidase CueO had previously been demonstrated to exhibit phenoloxidase activity and was implicated in intrinsic copper resistance in Escherichia coli. Catecholates can potentially reduce Cu(II) to the prooxidant Cu(I). In this report we provide evidence that CueO protects E. coli cells by oxidizing enterobactin, the catechol iron siderophore of E. coli, in the presence of copper. In vitro, a mixture of enterobactin and copper was toxic for E. coli cells, but the addition of purified CueO led to their survival. Deletion of fur resulted in copper hypersensitivity that was alleviated by additional deletion of entC, preventing synthesis of enterobactin. In addition, copper added together with 2,3-dihydroxybenzoic acid or enterobactin was able to induce a Phi(cueO-lacZ) operon fusion more efficiently than copper alone. The reaction product of the 2,3-dihydroxybenzoic acid oxidation by CueO that can complex Cu(II) ions was determined by gas chromatography-mass spectroscopy and identified as 2-carboxymuconate. |
| File Format | PDF HTM / HTML |
| DOI | 10.1128/JB.186.17.5826-5833.2004 |
| PubMed reference number | 15317788 |
| Journal | Medline |
| Volume Number | 186 |
| Issue Number | 17 |
| Alternate Webpage(s) | http://jb.asm.org/content/186/17/5826.full.pdf |
| Alternate Webpage(s) | http://www.k-state.edu/bmb/labs/pek/EPrints/GrassetalJBacty04.pdf |
| Alternate Webpage(s) | https://doi.org/10.1128/JB.186.17.5826-5833.2004 |
| Journal | Journal of bacteriology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
