NDLI: Expression of AQP3 protein in hAECs is regulated by Camp-PKA-CREB signalling pathway.

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Expression of AQP3 protein in hAECs is regulated by Camp-PKA-CREB signalling pathway.

Content Provider	Semantic Scholar
Author	Hua, Ying Yan Ding, Shengdi Zhang, Wenwen Zhou, Qingfeng Ye, Weijing Chen, Miaomiao Zhu, Xueqiong
Copyright Year	2015
Abstract	Previous studies by others and our group have demonstrated the expression of AQP3 protein in human chorioamniotic membranes. Here, we investigated whether cyclic adenosine monophosphate (cAMP) up-regulation of aquaporin 3 (AQP3) protein expression in human amniotic epithelial cells (hAECs) is mediated by protein kinase A (PKA) dependent or independent pathway. Cells were treated with various concentrations of Forskolin, SP-cAMP, H-89 at various time intervals or optimal concentration of Forskolin in combination with H-89 in the blocking experiments. Forskolin significantly increased cAMP levels and the expression of PKA, p-CREB and AQP3. SP-cAMP had similar effects. H-89 inhibited PKA, p-CREB and AQP3 protein expression, and attenuated the stimulatory effect of Forskolin. These results show that the AQP3 protein expression in hAECs was regulated by cAMP-PKA-CREB signal pathway. A relatively short biological half life of AQP3 renders its rapid responsiveness to regulation.
File Format	PDF HTM / HTML
DOI	10.2741/4357
PubMed reference number	25961543
Journal	Medline
Volume Number	20
Alternate Webpage(s)	https://www.bioscience.org/fbs/getfile.php?FileName=/2015/v20/af/4357/4357.pdf
Journal	Frontiers in bioscience
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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