Receptors for insulin-like growth factor-I in plasma membranes isolated from bovine mesenteric arteries.

Content Provider	Semantic Scholar
Author	Bornfeldt, Karin E. Arnqvist, Hans J. Dahlkvist, Hans Skottner, Anna Wikberg, Jarl E. S.
Copyright Year	1988
Abstract	Binding of IGF-I to plasma membranes from bovine mesenteric arteries was studied. The maximal specific binding of IGF-I was found to be 7.4 +/- 1.7% of total 125I-IGF-I added to the incubation medium. Unlabelled IGF-I displaced 125I-IGF-I with an IC50 value of 0.5 nmol/l and a maximal displacement of 64.2 +/- 2.8% of total binding. The potency of insulin to displace 125I-IGF-I was 100-1000-fold lower. Cross-linking of 125I-IGF-I to the receptor with disuccinimidyl suberate, followed by SDS-polyacrylamide gel electrophoresis under reducing conditions showed an IGF-I binding protein with a molecular weight of 146,000 Dalton. In summary, we have shown the presence of receptors for IGF-I in plasma membranes isolated from macrovessels. The binding characteristics and the size of the binding unit were found to be similar to those of the IGF-I receptor found in cultured vascular smooth muscle cells. Furthermore, insulin at high concentrations was found to interact with the IGF-I receptor.
Starting Page	1
Ending Page	7
Page Count	7
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://eje.bioscientifica.com/downloadpdf/journals/eje/117/4/acta_117_4_002.pdf
PubMed reference number	2968745v1
Volume Number	117
Issue Number	4
Journal	Acta endocrinologica
Language	English
Access Restriction	Open
Subject Keyword	Arterial system Bos taurus Cattle Gel Electrophoresis (lab technique) Growth Factor Inhibitory Concentration 50 Mesenteric Arteries Mesentery Molecular Weight Muscle, Smooth, Vascular Myocytes, Smooth Muscle Nanomole/liter Plasma membrane Polyacrylamide Gel Electrophoresis Psychologic Displacement Smooth muscle (tissue) Somatomedins dalton disuccinimidyl suberate polyacrylamide gels receptor
Content Type	Text
Resource Type	Article