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Site of fluoride accumulation in navel orange leaves.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Chang, C. W. Thompson, Charlotte R. |
| Copyright Year | 1966 |
| Abstract | Fluoride-polluted navel orange leaves, Citrus sinensis (Linn.) Osbeck, were fractionated into the subcellular components in hexane/carbon tetrachloride mixtures having various densities. Fluoride was determined at each fraction. Analyses were also made for the subcellular distribution of chlorophyll, nitrogen, and DNA to assess the extent of cross-contamination of each component. The fraction containing cell wall, nuclei, and partly broken cells apparently contained a major amount of fluoride. However, if allowance was made for the cross-contamination of chloroplasts and chloroplast fragments, the fraction of chloroplasts was found to be the site of the highest fluoride accumulation. When each particulate component was washed with water after drying, the combined washings contained more than 50% of the total fluoride of the isolated fractions. The usual method of subcellular fractionation with aqueous solvent shifted the major site of fluoride accumulation from the fraction of chloroplasts to that of the supernatant. |
| Starting Page | 139 |
| Ending Page | 140 |
| Page Count | 2 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.plantphysiol.org/content/plantphysiol/41/2/211.full.pdf |
| PubMed reference number | 5908632v1 |
| Volume Number | 41 |
| Issue Number | 2 |
| Journal | Plant physiology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Authorization documentation Carbon Tetrachloride Cell Wall Chlorophyll Chloroplasts Citrus aurantium Citrus plant Citrus sinensis Contain (action) Fluorides Greater Than Particulate (substance) Supernatant Umbilicus (Anatomy) density mixture n-hexane |
| Content Type | Text |
| Resource Type | Article |
