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An Accurate and Effective Method for Measuring Osimertinib by UPLC-TOF-MS and Its Pharmacokinetic Study in Rats
| Content Provider | Semantic Scholar |
|---|---|
| Author | Dong, Song-Tao Li, Ying Yang, Hao-Tian Wu, Yin Lin Li, Ya-jing Ding, Cong-Yang Meng, Lu Dong, Zhan-Jun Zhang, Yuan Bo |
| Copyright Year | 2018 |
| Abstract | Osimertinib, a new-generation inhibitor of the epidermal growth factor, has been used for the clinical treatment of advanced T790M mutation-positive tumors. In this research, an original analysis method was established for the quantification of osimertinib by ultra-performance liquid chromatography with time of flight mass spectrometry (UPLC-TOF-MS) in rat plasma. After protein precipitation with acetonitrile and sorafinib (internal standard, IS), they were chromatographed through a Waters XTerra MS C18 column. The mobile phase was acetonitrile and water (including 0.1% ammonia). The relative standard deviation (RSD) of the intra- and inter-day results ranged from 5.38 to 9.76% and from 6.02 to 9.46%, respectively, and the extraction recovery and matrix effects were calculated to range from 84.31 to 96.14% and from 91.46 to 97.18%, respectively. The results illustrated that the analysis method had sufficient specificity, accuracy and precision. Meanwhile, the UPLC-TOF-MS method for osimertinib was successfully applied into the pharmacokinetics of SD rats. |
| Starting Page | 1845 |
| Ending Page | 1858 |
| Page Count | 14 |
| File Format | PDF HTM / HTML |
| PubMed reference number | 30404182v1 |
| Alternate Webpage(s) | https://doi.org/10.3390/molecules23112894 |
| DOI | 10.3390/molecules23112894 |
| Journal | Molecules |
| Volume Number | 23 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
