Binding of 5′-O-(2-thiodiphosphate) to rat brain membranes is prevented by diadenosine tetraphosphate and correlates with ecto-nucleotide pyrophosphatase phosphodiesterase 1 (NPP1) activity

Content Provider	Semantic Scholar
Author	Oaknin, Sol Rodríguez-Ferrer, C. R. Ramos, Antonio Aguilar, José Santiago Rotllán, Pedro
Copyright Year	2008
Abstract	The distribution of binding sites for [(35)S]5'-O-(2-thiodiphosphate) ([(35)S]ADPbetaS), a radioligand of P2Y(1,12,13) receptors, and of ecto-nucleotide pyrophosphatase phosphodiesterase activity were analyzed in the rat forebrain. Binding sites for the radilogand are widespreadly distributed in the rat forebrain, showing the highest density in hypothalamus. K(d) values were in the range 1-2 nM. Diadenosine tetraphosphate (Ap(4)A) and diethenoadenosine tetraphosphate, epsilon-(Ap(4)A), displaced the radioligand, indicating dinucleotide binding to ADPbetaS-recognizing P2Y receptors. Activity ecto-nucleotide pyrophosphatase phosphodiesterase 1 (NPP1), able to hydrolyze Ap(4)A and other diadenosine polyphosphates, is also widely distributed through the rat forebrain, with the highest activity in hypothalamus. These results suggests that Ap(4)A signalling mediated by P2Y(1,12,13) receptors and enzymatically regulated by NPP1 activity may be particularly important in hypothalamus and add new support for neurotransmitter/neuromodulatory functions of diadenosine polyphosphates in brain.
Starting Page	25
Ending Page	29
Page Count	5
File Format	PDF HTM / HTML
DOI	10.1016/j.neulet.2007.11.056
PubMed reference number	18162317
Journal	Medline
Volume Number	432
Alternate Webpage(s)	https://api.elsevier.com/content/article/pii/S0304394007012542
Alternate Webpage(s)	https://www.sciencedirect.com/science/article/pii/S0304394007012542?dgcid=api_sd_search-api-endpoint
Alternate Webpage(s)	https://doi.org/10.1016/j.neulet.2007.11.056
Journal	Neuroscience Letters
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article