Loading...
Please wait, while we are loading the content...
Similar Documents
Diagnostic accuracy of methods for detecting Anaplasma marginale infection in lactating dairy cattle of Puerto Rico.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Fosgate, Geoffrey T. Urdaz-RodrÃguez, José H. Dunbar, Mark D. Rae, D. Owen Donovan, G. Arthur Melendez, Pedro Dobek, Georgina L. Alleman, Arthur R. |
| Copyright Year | 2010 |
| Abstract | Bovine anaplasmosis (BA) is a hemoparasitic disease of great importance in cattle within the tropical and subtropical regions of the world. Control programs for BA require accurate diagnostic assays but validation can be challenging because the true disease status of all animals is frequently not known with certainty. The objective of this study was to estimate the accuracy of assays for detection of Anaplasma marginale infection in lactating dairy cattle of Puerto Rico using Bayesian methods without a perfect reference test. There were 2,331 cattle with complete diagnostic results sampled from 79 herds, and the prevalence of BA was estimated as 22% (95% probability interval [PI]: 19-25%). The sensitivity (Se) and specificity (Sp) of a major surface protein 5 competitive enzyme-linked immunosorbent assay (MSP-5 cELISA) were estimated as 99% (95% PI: 96-100%) and 89% (95% PI: 87-92%), respectively. The Se and Sp of a quantitative polymerase chain reaction (qPCR) were 67% (95% PI: 60-74%) and 99% (95% PI: 99-100%). The Se and Sp of a card agglutination test were 34% (95% PI: 29-39%) and 99% (95% PI: 99-100%). Area under the receiver-operating characteristic curve for the MSP-5 cELISA was 0.748 (95% PI: 0.71-0.79). The MSP-5 cELISA appears to be the test of choice for screening cattle for subclinical BA based on the high estimated Se, rapidity of results, relative low cost, and ease of standardization. |
| Starting Page | 24 |
| Ending Page | 33 |
| Page Count | 10 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://repository.up.ac.za/bitstream/handle/2263/15556/Fosgate_Diagnostic(2010).pdf;jsessionid=A786C47BAF6F6EAE5A5B62F511E5CE28?sequence=1 |
| PubMed reference number | 20224076v1 |
| Volume Number | 22 |
| Issue Number | 2 |
| Journal | Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Agglutination Tests Anaplasma marginale Bovine Anaplasmosis Dairy Eighty Nine Enzyme-Linked Immunosorbent Assay Membrane Proteins Ninety Nine Platelet aggregation.epinephrine induced:RelACnc:Pt:PRP:Qn Polymerase Chain Reaction Sampling - Surgical action Seventy Nine Status Epilepticus |
| Content Type | Text |
| Resource Type | Article |
