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Protective effects of propofol against hydrogen peroxide-induced oxidative stress in human kidney proximal tubular cells
| Content Provider | Semantic Scholar |
|---|---|
| Author | Lee, Young Moo Shin, Jin Woo Lee, Eun Ho Moon, Youngjin Seo, Young Joo Kim, Ji Yeon Kim, Joung Uk |
| Copyright Year | 2012 |
| Abstract | BACKGROUND We investigated the protective effects of propofol in the HK-2 cell line of human kidney proximal tubular cells against hydrogen peroxide (H(2)O(2))-induced oxidative stress. METHODS After pretreatment with different concentrations of propofol (0 µM, 10 µM, 25 µM and 50 µM) for 30 minutes, HK-2 cells were exposed to 8 mM H(2)O(2) for 4 hours. Cell death was assessed by measuring the percentage of lactate dehydrogenase (LDH) release and by counting viable cells. The nature of cell death was assessed by doubles-taining cells with fluorescein isothiocyanate-labeled Annexin V and propidium iodide, and then analyzing the cells using flow cytometry. RESULTS After exposure to 8 mM H(2)O(2) for 4 hours, the percentage of LDH release was 45.1 ± 4.2% and the number of viable HK-2 cells was 5.2 ± 6.0%. Pretreatment with propofol suppressed H(2)O(2)-induced LDH release in a concentration-dependent manner, reducing the percentage of LDH release to 38.1 ± 5.6%, 33.5 ± 6.3%, and 26.2 ± 3.8% of the controls at 10 µM, 25 µM and 50 µM propofol, respectively. Numbers of viable cells increased following propofol pretreatment, with 11.4 ± 10.9%, 19.5 ± 16.1%, and 32.4 ± 23.3% cell survival rates after pretreatment with 10 µM, 25 µM and 50 µM propofol, respectively. Analyses of flow cytometry showed that the propofol pretreatment decreased the percentage of necrotic and late apoptotic cells. CONCLUSIONS Propofol protects HK-2 human kidney proximal tubular cells against H(2)O(2)-induced oxidative stress. |
| Starting Page | 441 |
| Ending Page | 446 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| DOI | 10.4097/kjae.2012.63.5.441 |
| PubMed reference number | 23198039 |
| Journal | Medline |
| Volume Number | 63 |
| Alternate Webpage(s) | https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/47/df/kjae-63-441.PMC3506855.pdf |
| Journal | Korean journal of anesthesiology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
