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Effect of potential-dependent potassium uptake on production of reactive oxygen species in rat brain mitochondria
| Content Provider | Semantic Scholar |
|---|---|
| Author | Akopova, O. V. Kolchinskaya, L. I. Nosar, V. I. Bouryi, V. A. Mankovska, I. G. Sagach, V. F. |
| Copyright Year | 2014 |
| Abstract | The effect of potential-dependent potassium uptake on reactive oxygen species (ROS) generation in mitochondria of rat brain was studied. It was found that the effect of K+ uptake on ROS production in the brain mitochondria under steady-state conditions (state 4) was determined by potassium-dependent changes in the membrane potential of the mitochondria (ΔΨm). At K+ concentrations within the range of 0–120 mM, an increase in the initial rate of K+-uptake into the matrix resulted in a decrease in the steady-state rate of ROS generation due to the K+-induced depolarization of the mitochondrial membrane. The selective blockage of the ATP-dependent potassium channel (KATP+-channel) by glibenclamide and 5-hydroxydecanoate resulted in an increase in ROS production due to the membrane repolarization caused by partial inhibition of the potential-dependent K+ uptake. The ATP-dependent transport of K+ was shown to be ∼40% of the potential-dependent K+ uptake in the brain mitochondria. Based on the findings of the experiments, the potential-dependent transport of K+ was concluded to be a physiologically important regulator of ROS generation in the brain mitochondria and that the functional activity of the native KATP+ -channel in these organelles under physiological conditions can be an effective tool for preventing ROS overproduction in brain neurons. |
| Starting Page | 44 |
| Ending Page | 53 |
| Page Count | 10 |
| File Format | PDF HTM / HTML |
| DOI | 10.1134/S0006297914010076 |
| PubMed reference number | 24512663 |
| Journal | Medline |
| Volume Number | 79 |
| Alternate Webpage(s) | https://page-one.springer.com/pdf/preview/10.1134/S0006297914010076 |
| Alternate Webpage(s) | https://doi.org/10.1134/S0006297914010076 |
| Journal | Biochemistry (Moscow) |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
