Proximity hybridization regulated DNA biogate for sensitive electrochemical immunoassay.

Content Provider	Semantic Scholar
Author	Ren, Kewei Zhang, Yue Yan, Feng Ju, Huangxian
Copyright Year	2014
Abstract	An electrochemical DNA biogate was designed for highly sensitive homogeneous electrochemical immunoassay by combining target-induced proximity hybridization with a mesoporous silica nanoprobe (MSN). The electroactive methylene blue (MB) was sealed in the inner pores of MSN with single-stranded DNA. In the presence of target protein and two DNA-labeled antibodies, the formed proximate complex could hybridize with the DNA strand to form a rigid double-stranded structure and thus open the biogate, which led to the release of MB entrapped in the MSN. The target protein-dependent amount of released MB could be conveniently monitored with a screen-printed carbon electrode. Moreover, the detachment process of MB could be further amplified with an in situ enzymatic recycling binding of the proximate complex with the single-stranded DNA. Using prostate-specific antigen as a model target, the proposed assay showed a wide detection range from 0.002 to 100 ng mL(-1) with a detection limit of 1.3 pg mL(-1). This strategy was simple and universal for various analytes with different affinity ligands. This method possessed great potential for convenient point-of-care testing and commercial application.
File Format	PDF HTM / HTML
DOI	10.1021/ac5012377
PubMed reference number	24965810
Journal	Medline
Volume Number	86
Issue Number	15
Alternate Webpage(s)	http://sklac.nju.edu.cn/hxju/lunwenlunzhu/paper2014/469%20Anal%20Chem%20Ren%20KW.pdf
Journal	Analytical chemistry
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article