NDLI: Talin1 is critical for force-dependent reinforcement of initial integrin–cytoskeleton bonds but not tyrosine kinase activation

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Talin1 is critical for force-dependent reinforcement of initial integrin–cytoskeleton bonds but not tyrosine kinase activation

Content Provider	Semantic Scholar
Author	Giannone, Grégory Jiang, Guoying Sutton, Deborah H. Critchley, David R. Sheetz, Michael P.
Copyright Year	2003
Abstract	Cells rapidly transduce forces exerted on extracellular matrix contacts into tyrosine kinase activation and recruitment of cytoskeletal proteins to reinforce integrin-cytoskeleton connections and initiate adhesion site formation. The relationship between these two processes has not been defined, particularly at the submicrometer level. Using talin1-deficient cells, it appears that talin1 is critical for building early mechanical linkages. Deletion of talin1 blocked laser tweezers, force-dependent reinforcement of submicrometer fibronectin-coated beads and early formation of adhesion sites in response to force, even though Src family kinases, focal adhesion kinase, and spreading were activated normally. Recruitment of vinculin and paxillin to sites of force application also required talin1. FilaminA had a secondary role in strengthening fibronectin-integrin-cytoskeleton connections and no role in stretch-dependent adhesion site assembly. Thus, force-dependent activation of tyrosine kinases is independent of early force-dependent structural changes that require talin1 as part of a critical scaffold.
Starting Page	409
Ending Page	419
Page Count	11
File Format	PDF HTM / HTML
PubMed reference number	14581461
Volume Number	163
Journal	The Journal of cell biology
Language	English
Access Restriction	Open
Subject Keyword	Bead Dosage Form Cytoskeleton ErbB Receptors Extracellular Matrix Focal Adhesions Optical Trap PXN gene Protein Tyrosine Kinase VCL gene physical hard work
Content Type	Text
Resource Type	Article

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