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Propagación in vitro de Solanum dolichosepalum (Solanaceae) / In vitro Propagation of Solanum dolichosepalum (Solanaceae)
| Content Provider | Semantic Scholar |
|---|---|
| Author | Burgos, Camilo Andrés Cárdenas |
| Copyright Year | 2016 |
| Abstract | Resumen Se establecio un protocolo para micropropagacion de Solanum dolichosepalum a partir de semillas desinfectadas con NaOCl y germinadas en MS/4. La multiplicacion de microtallos se realizo por segmentos nodales, evaluando el efecto de la posicion del segmento nodal, concentracion de AIB y BA, concentracion de sales MS en presencia de AIB, concentracion de sales MS y sacarosa en presencia de carbon activado, diferentes medios de cultivo y concentracion de carbon activado sobre la longitud total de microtallos, promedio de yemas neoformadas, porcentaje de microtallos con callo basal y porcentaje de microtallos enraizados. Despues de 20 dias de cultivo en MS/4 el 100% de las semillas cultivadas desarrollaron plantulas de apariencia normal. La multiplicacion de microtallos fue exitosa en medio H con 20% de sacarosa suplementado con 0,02 mg/L de ANA y de BA, despues de 30 dias de subcultivo los microtallos desarrollados alcanzaron 4 cm de longitud, formaron 4 yemas axilares, el 3 % formo callo basal y el 100% presento enraizamiento. La aclimatizacion de plantulas se realizo exitosamente en una mezcla de tierra, capote y arena (3:2:1). Este protocolo forma parte de un programa de produccion de material injertado de lulo de castilla para establecimiento de huertos comerciales. Abstract A protocol for micropropagation of Solanum dolichosepalum was established from seeds disinfected with NaOCl and germinated in MS/4. Multiplication of microshoots was performed by nodal segments evaluating the effect of the nodal segment position, BA and IBA concentrations, MS salts concentration in presence of AIB, MS salts and sucrose concentration in the presence of activated charcoal, different culture media, and activated charcoal concentration on the total length of microshoots, average of newly formed buds, percentage of microshoots with basal callus and percentage of rooted microshoots. After 20 days of culture in MS/4 100% of cultured seeds developed plantlets with normal appearance. Microshoots multiplication was successful in medium H with 2% sucrose supplemented with 0,02 mg L-1 NAA and BA; after 30 days of subculture, the developed microshoots reached 4 cm in length, formed four axillary buds, 3% formed basal callus and 100% developed roots. Plantlets acclimatization was successfully performed on a mixture of soil, mulch and sand (3:2:1). This protocol is part of a program to produce grafted material of “Lulo de Castilla” for establishment of commercial orchards. |
| Starting Page | 9 |
| Ending Page | 22 |
| Page Count | 14 |
| File Format | PDF HTM / HTML |
| DOI | 10.19053/01217488.v7.n2.2016.4122 |
| Alternate Webpage(s) | http://www.scielo.org.co/pdf/cide/v7n2/v7n2a01.pdf |
| Alternate Webpage(s) | https://doi.org/10.19053/01217488.v7.n2.2016.4122 |
| Volume Number | 7 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
