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Antimicrobial activity and molecular characterization of endophytic fungi strain isolated from dahlia ( Dahlia variabilis )
| Content Provider | Semantic Scholar |
|---|---|
| Author | Saryono Hendris, Sefni Fitriyah, Dina Jose, Christine Nugroho, Titania Tjandrawati Ardhi, Aulia |
| Copyright Year | 2015 |
| Abstract | Dahlia variabilis has many beneficial effects to hu man health, such as antibacterial and antifungi age nt. This research aimed to discover the potency of endophyti c microbesisolated from Dahlia as antimicrobial age nts. Screenings were carried out using agar diffusion me thod against pathogens with concentration of 50μl/d isc. Based on our previous study, four isolates of endophytic fungi were isolated and characterized morphological ly s follows, LBKURCC40 identified as Monilia sp., LBKURCC41 iden tified as Aureobasidium sp., LBKURCC42 identified a s Moniliella sp., and LBKURCC42 identified as Sporoth ix sp. The inhibition zone of amoxsan was 12.55 ±0 .38 mm and ketoconazole was 17.67±0.57 mm. LBKURCC41 showe d th most significant inhibition zones against Eschericia coli and Staphylococcus aureus which tho se were 19.33±1.15 mm and 17.00±1.00 mm respectivel y. LBKURCC41 was then characterized genetically. The m olecular weight of chromosomal DNA of LBKURCC41 obtained was 11,706 bp. Its rDNA was amplified with PCR using ITS5 and ITS4 as DNA primers and the res ult of phylogenetic analysis at ITS-1, ITS-2 and 5.8S rDNA showed that LBKURCC41 was Fusarium oxysporum with 99% similarity, which was different from previous morph ological characterization. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.jocpr.com/articles/antimicrobial-activity-and-molecular-characterization-of-endophytic-fungi-strain-isolated-from-dahlia-dahlia-variabilis.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
