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EPR Detection of the Superoxide Free Radical with the Nitrone Spin Traps
| Content Provider | Semantic Scholar |
|---|---|
| Copyright Year | 2012 |
| Abstract | acterizing free radicals in chemistry, biology and medicine, is detection by EPR spectroscopy. However, due to their high reactivity and short half-lives, direct EPR detection of many free radicals (e.g., superoxide, hydroxyl radical, alkyl radicals, etc.) is virtually impossible in solution at room temperature. Spin trapping is a technique developed in the late 1960s in which a nitrone or nitroso compound reacts with a target free radical to form a stable and identifiable free radical that is detected by EPR spectroscopy. The spin trapping technique involves the addition of the reactive free radical across the double bond of a diamagnetic ‘‘spin trap’’ to form a much more stable free radical (a ‘‘radical adduct’’) which can then be measured with EPR: other nitrone spin traps. It is very redox inert. DMPO forms radical adducts with O-, C-, N-, S-centered radicals that have very distinguishable EPR spectra. This allows the researcher to identify the type of free radical that was formed in a given reaction. This is not the case for other spin traps such as α-phenyl-N-tert-butylnitrone (PBN) where the EPR spectra for the radical adducts are nearly identical regardless of the radical trapped. The identity of a DMPO radical adduct is then identified using references from a web search of related scientific literature. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.bruker.com/fileadmin/user_upload/8-PDF-Docs/MagneticResonance/EPR_brochures/superoxide.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
