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Identification of a calcium-dependent calmodulin-binding domain in Xenopus membrane skeleton protein 4.1.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Kelly, G. M. Zelus, Bruce D. Moon, Randall T. |
| Copyright Year | 1991 |
| Abstract | Xenopus membrane skeleton protein 4.1 is expressed constitutively during embryonic development and accumulates to high levels within the retina during normal morphogenesis. There exists a high degree of amino acid identity between Xenopus protein 4.1 and human protein 4.1, suggesting that the mechanisms known to modulate the function(s) of human protein 4.1 may also serve to regulate Xenopus protein 4.1. Calmodulin (CaM) is one regulatory protein known to affect membrane-cytoskeletal interactions. An in vitro binding assay was used to test the ability of Xenopus protein 4.1 to bind CaM. Two independent approaches, involving protein 4.1 synthesized in vitro from synthetic RNA or a partial length protein 4.1 fusion protein expressed in Escherichia coli, demonstrate calcium-dependent, CaM binding. Both approaches demonstrate that the CaM-binding site is within the amino-terminal region of Xenopus protein 4.1. Results of this calmodulin binding activity suggest a possible regulatory mechanism by which calcium and calmodulin may affect the function of protein 4.1 during development. |
| File Format | PDF HTM / HTML |
| PubMed reference number | 2061322 |
| Journal | Medline |
| Volume Number | 266 |
| Issue Number | 19 |
| Alternate Webpage(s) | http://www.jbc.org/content/266/19/12469.full.pdf |
| Journal | The Journal of biological chemistry |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
