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Title Electrochemical Surface-Enhanced Raman Spectroscopy ( EC-SERS ) for Bio-analysis on Silver-sputtered Screen-printed Electrode
| Content Provider | Semantic Scholar |
|---|---|
| Author | Zhu, Zicheng |
| Abstract | of Thesis Electrochemical Surface-enhanced Raman spectroscopy (EC-SERS) is promising analytical tool for monitoring and rapid detection of analytes with high sensitivity. Compared with non-electrochemical SERS, EC-SERS takes the advantages of manipulate analyte on the surface through electrochemical modulation, which provides the enhanced signal and more selective peaks. The original method has been proposed for rapid and highly productive fabricating SERS-active screen-printed electrode (SPE) by sputtering deposition which is introduced. Two kinds of working electrodes of SPE which were carbon electrode and gold electrode were successfully deposited by silver nanoparticles. Besides, the sputtering experiments of different substrates (paper, glass epoxy and polyethylene terephthalate) of the SPE were also conducted. The comparison of SERS enhancement of rhodamine 6G (R6G) on different kinds of SERS-active SPEs was introduced. In addition, the relationship between the surface roughness (observed through AFM and SEM images) and SERS enhancement has been investigated. Compared with the classical citrate reduction method, sputtering method guaranteed low SERS background signal and maximum of surface absorption for target analytes because there was no other material except pure silver directly deposited on the working electrode. These results demonstrated the applicability of this mass producible fabrication method for producing SERS-active SPEs, as well as, highlighting the future potential of commercial bio-applications. For application of EC-SERS technology to biosensors, uric acid and aminoglutethimide (AGI) have been investigated. For the latter application, a linear dependence occurs in the range of 1 x 10-5 M to 2 x 10-7 M. The limit of detection (LOD) is 40 ng/mL and the R squared of the linear curve is 0.98. Compared with non-electrochemical SERS, the signal not only more selective peak signals appeared but also increasing signal intensities of the target analyte could be observed through the EC-SERS spectra. The benefits brought by this method include simple experimental process, fast detection time and high sensitivity. In the future, the EC-SERS sensor may become a useful tool for rapid and routine analysis in patient biomarkers and anti-doping detection for on-site use. (高空間分解能カラーナノイメージングのための金属ナノロッド鎖プラズモニックナノレンズ) |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://ir.library.osaka-u.ac.jp/repo/ouka/all/72373/30366_Abstract.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
