A novel ratiometric fluorescent probe for selective detection of bisulfite in living cells

Content Provider	Semantic Scholar
Author	Chen, Zhen Chen, Fengzao Sun, Yuanchao Liu, Heng He, Hanping Zhang, Xiuhua Wang, Shengfu
Copyright Year	2017
Abstract	A new type of ratiometric fluorescent probe, 8-(diethylamino)-2-methyl-4-oxo-4H-pyrano[2,3-b]chromen-10-ylium tetrafluoroborate (APCT), is reported. A new type of ratiometric fluorescent probe, 8-(diethylamino)-2-methyl-4-oxo-4H-pyrano[2,3-b]chromen-10-ylium tetrafluoroborate (APCT), is reported. This probe can successfully detect bisulfite anions with a detection limit of 6.1 × 10−7 M based on Michael-type addition reaction in pH = 7.4 Tris buffer solution containing 70% DMSO (v/v). The response time of the probe is approximately 5 min. Upon treating with bisulfite anions, the probe exhibits an obvious blue shift from 560 nm to 510 nm and the fluorescence intensity ratio at 510 nm and 560 nm (I510/I560) displays a good linear relationship with the concentration of HSO3− in the range of 20–120 μM. Additionally, the preliminary cell imaging experiments using HeLa cells demonstrate that the probe can be used to detect intracellular bisulfite anions.
Starting Page	2573
Ending Page	2577
Page Count	5
File Format	PDF HTM / HTML
DOI	10.1039/C6RA27347E
Volume Number	7
Alternate Webpage(s)	http://pubs.rsc.org/en/content/articlepdf/2017/ra/c6ra27347e
Alternate Webpage(s)	https://doi.org/10.1039/C6RA27347E
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article