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Optimization of Fermentation Conditions for Laccase Production by Recombinant Pichia pastoris GS115-LCCA Using Response Surface Methodology and Its Application to Dye Decolorization
| Content Provider | Semantic Scholar |
|---|---|
| Author | Xie, Jingcong Zhao, Linguo Xue, Qiuxia Pei, Jimin |
| Copyright Year | 2013 |
| Abstract | In this work, recombinant Pichia pastoris GS115-lccA was cultured and expressed with the highest laccase activity of 2357 U/L with ABTS (2, 2'-nazinobis-(3-ethylbenzthiazoline-6-sulphonate)) as reacting substrate. To achieve the higher laccase activity, multiple key factors were screened (using a Plackett-Burman design of experiments methodology) related to the fermentation conditions for producing GS115-lccA. Subsequently, a Box-Behnken response surface methodology was employed for further optimization. The optimum fermentation conditions of the fermentation were obtained as follows: 0.603% methanol added into the culture every 24 h, medium optimal initial pH 7.1, and liquid medium volume of 20.4% provided the highest enzyme activity of 5235 U/L. The decolorization experiments of dyes (Reactive Blue KN-R and Acid Red 35) were carried with the laccase cultured by recombinant P. pastoris GS115-lccA. This purified enzyme showed excellent decolorization capacity. After 24 h, the decolorization of Reactive Blue KN-R with 100 mg/L at 50 °C, pH 4.5 using 20 mM acetate buffer with 2 U/mL purified enzyme was 91.33%, and for Acid Red 35, the decolorization was 78.96%. All results suggested that this laccase may be suitable for the wastewater treatment of similar azo and anthraquinone dyes from the deinking and dyeing industries. |
| Starting Page | 4072 |
| Ending Page | 4087 |
| Page Count | 16 |
| File Format | PDF HTM / HTML |
| DOI | 10.15376/biores.8.3.4072-4087 |
| Volume Number | 8 |
| Alternate Webpage(s) | https://bioresources.cnr.ncsu.edu/BioRes_08/BioRes_08_3_4072_Li_XZXP_Opt_Ferment_Laccase_Pichia_LCCA_RSM_Dye_3983.pdf |
| Alternate Webpage(s) | https://doi.org/10.15376/biores.8.3.4072-4087 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
