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Electrophoretic analysis of hemoglobin and isozymes in individual vertebrate cells.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Rosenberg, Martin Goldstern Hajime Machida Ivo G. |
| Copyright Year | 1970 |
| Abstract | Electrophoretic patterns of enzymes in single vertebrate erythrocytes may be visualized with the use of capillary tube polyacrylamide columns and stains which amplify the enzymatic activity. The following points have thereby been demonstrated:1. Peripheral blood of metamorphosing bullfrogs (Rana catesbeiana) contains two molecular forms of hemoglobin: embryonic (tadpole) and adult (frog). Only one of these forms is found in each erythrocyte.2. Human umbilical cord blood contains fetal and adult hemoglobins; both forms may be found in individual erythrocytes, in varying proportions.3. Both lactate dehydrogenase isozymes found in whole blood of bullfrogs and humans are found in individual erythrocytes, but the proportions differ from cell to cell. |
| Starting Page | 447 |
| Ending Page | 450 |
| Page Count | 4 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.pnas.org/content/67/1/32.full.pdf |
| PubMed reference number | 5272321v1 |
| Volume Number | 67 |
| Issue Number | 1 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Hemoglobin Lactate Dehydrogenase Lactic acid Rana catesbeiana TUBE, COLLECTION, CAPILLARY BLOOD Umbilical Cord Blood Umbilical cord structure Umbilicus (Anatomy) polyacrylamide |
| Content Type | Text |
| Resource Type | Article |
