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SNAIL induces epithelial-to-mesenchymal transition and cancer stem cell-like properties in aldehyde dehydroghenase-negative thyroid cancer cells.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Yasui, Kazuaki Shimamura, Mika Mitsutake, Norisato Nagayama, Yuji |
| Copyright Year | 2013 |
| Abstract | BACKGROUND Epithelial-to-mesenchymal transition (EMT) is thought to play a critical role in the invasion and metastasis of cancer and to be associated with cancer stem cell (CSC) properties. It is not clear if there is a link between EMT and CSCs in thyroid cancers. We therefore investigated the CSC properties of thyroid cancers that underwent EMT. METHOD To induce EMT (spindle-like cell morphology, loss and acquisition of expression of an epithelial marker E-cadherin and a mesenchymal marker vimentin respectively) in an epithelial-type thyroid cancer cell line ACT-1, we used transforming growth factor-β (TGF-β), BRAF(V600E), and/or Snail homolog 1 (SNAI1, also known as SNAIL). CSC properties were analyzed with assays for cell proliferation, chemosensitivity, in vitro and in vivo tumor formation ability, cell surface antigens, and intracellular aldehyde dehydrogenase (ALDH; a known CSC marker) activities. RESULTS EMT was induced most efficiently by SNAIL (ACT-SNAIL cells), whereas TGF-β and BRAF(V600E) were less efficient. ACT-SNAIL cells showed slightly but significantly enhanced tumor formation ability in an in vitro sphere assay (approximately 3-fold) but not an in vivo subcutaneous tumor growth assay, and showed comparable chemosensitivity compared with the parental ACT-1 cells. However, of interest, although the in vitro sphere-formation ability of ALDH(+) cells was almost unchanged after SNAIL induction, SNAIL overexpression induced much higher (approximately 14-fold) spheres in ALDH(-) cells. Thus, ALDH was no longer a CSC marker in ACT-SNAIL cells. CONCLUSIONS All these data indicate that EMT confers CSC properties in ALDH(-) cells and appears to influence the ability of ALDH to enrich CSCs. |
| Starting Page | 689 |
| Ending Page | 694 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://naosite.lb.nagasaki-u.ac.jp/dspace/bitstream/10069/36079/1/ISYK714_Yasui.pdf |
| Alternate Webpage(s) | http://naosite.lb.nagasaki-u.ac.jp/dspace/bitstream/10069/33911/1/Thy23_989.pdf |
| PubMed reference number | 23432420v1 |
| Alternate Webpage(s) | https://doi.org/10.1089/thy.2012.0319 |
| DOI | 10.1089/thy.2012.0319 |
| Journal | Thyroid : official journal of the American Thyroid Association |
| Volume Number | 23 |
| Issue Number | 8 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
