NDLI: Effects of insulin-like growth factor 1 on voltage-gated ion channels in cultured rat hippocampal neurons

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Effects of insulin-like growth factor 1 on voltage-gated ion channels in cultured rat hippocampal neurons

Content Provider	Semantic Scholar
Author	Xing, Changhong Yin, Yanling He, Xiangping Xie, Zuoping
Copyright Year	2006
Abstract	Insulin-like growth factor 1 (IGF-1) has important functions in the brain, including metabolic, neurotrophic, neuromodulatory, and neuroendocrine actions, and it is also prevents amyloid beta-induced death of hippocampal neurons. However, its functions on the voltage-gated ion channels in hippocampus remain uncertain. In the present study, we investigated the effects of IGF-1 on voltage-gated potassium, sodium, and calcium channels in the cultured rat hippocampal neurons using the whole-cell patch clamp recordings. Following incubation with different doses of IGF-1 for 24 h, a block of the peak transient A-type K+ currents amplitude (IC50: 4.425 ng/ml, Hill coefficient: 0.621) was observed. In addition, after the application of IGF-1, the amplitude of high-voltage activated Ca2+ currents significantly increased but activation kinetics did not significantly alter (V1/2: -33.45 +/- 1.32 mV, k = 6.16 +/- 1.05) compared to control conditions (V1/2: -33.19 +/- 2.28 mV, k = 7.26 +/- 1.71). However, the amplitude of Na+, K+, and low-voltage activated Ca2+ currents was not affected by the application of IGF-1. These data suggest that IGF-1 inhibits transient A-type K+ currents and enhances high-voltage-activated Ca2+ currents, but has no effects on Na+ and low-voltage-activated Ca2+ currents.
Starting Page	30
Ending Page	35
Page Count	6
File Format	PDF HTM / HTML
DOI	10.1016/j.brainres.2005.10.091
Alternate Webpage(s)	https://api.elsevier.com/content/article/pii/S0006899305015751
Alternate Webpage(s)	https://www.sciencedirect.com/science/article/pii/S0006899305015751?dgcid=api_sd_search-api-endpoint
PubMed reference number	16438944
Alternate Webpage(s)	https://doi.org/10.1016/j.brainres.2005.10.091
Journal	Medline
Volume Number	1072
Journal	Brain Research
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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