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Induced luteolysis in the primate: rapid loss of luteinizing hormone receptors.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Duncan, William Colin Illingworth, Peter J. Young, Faith M. Fraser, Hamish M. |
| Copyright Year | 1998 |
| Abstract | The molecular mechanisms involved in luteolysis are still unclear in the primate. This study aimed to investigate the effect of induced luteolysis on the ovarian luteinizing hormone (LH) receptor and the steroidogenic enzyme, 3beta-hydroxysteroid dehydrogenase (3beta-HSD) in the marmoset monkey. Luteolysis was induced in the mid-luteal phase either directly by systemic prostaglandin F2alpha (PGF2alpha), or indirectly by LH withdrawal using systemic gonadotrophin releasing hormone antagonist (GnRHant) treatment. The LH receptor was studied by isotopic mRNA in-situ hybridization and in-situ ligand binding and 3beta-HSD expression was studied using isotopic mRNA in-situ hybridization and immunohistochemistry. Induced luteolysis was associated with a reduction in the expression of LH receptor (P < 0.0001) and 3beta-HSD mRNA, closely followed by a reduction in the LH receptor (P < 0.05) and 3beta-HSD protein concentrations within 24 h. There were no differences in the findings whether luteolysis was induced with PGF2alpha or GnRHant. This study shows that disparate mechanisms to induce luteolysis in the primate result in an identical rapid loss of the LH receptor and 3beta-HSD. In conclusion, induced luteolysis leads to rapid loss of the steroidogenic pathway in luteal cells. |
| Starting Page | 79 |
| Ending Page | 81 |
| Page Count | 3 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/humrep/13/9/10.1093_humrep_13.9.2532/1/132532.pdf?Expires=1493247665&Key-Pair-Id=APKAIUCZBIA4LVPAVW3Q&Signature=dDfOLTDKOLVJKCXADTk6yum6dhbO6Y9VCmR4Vu0xpJRsGZtbULA7pb6doW3pgjxbqgwXN1Y1bcFexHqKupEVCR1oQPf6P9m3rvHWHFPO~0HABQ6HsR5g9OgdNq9dRLI2guadcGGgG0KKGaIBsDBHbUoWcIcCB2T~zIGmOj4wjMtscVEK39Q2g~6y5wAuMQqacTphmFLGr4lKj50~j7a37a4908j0j4x31tnU1r0SNnRgEfxn6hjIspnLs4DYkYDSXhmjPYat5rj9sXJoDndZpvM9eoSh3yXsarr2gqi18Wy7OnF2QxGroV2FHgKLMp6rDLVcLj7Xp2iTOTtDRUla4A__ |
| PubMed reference number | 9806279v1 |
| Volume Number | 13 |
| Issue Number | 9 |
| Journal | Human reproduction |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | 17-Hydroxysteroid Dehydrogenase Deficiency 3 beta-Hydroxysteroid dehydrogenase deficiency Callithrix Dinoprost Gonadorelin Gonadotropins Hydroxysteroid Dehydrogenases Hydroxysteroids In Situ Hybridization Ligands Luteal Phase Luteinizing Hormone Luteolysis Nucleic Acid Hybridization Primates Prostaglandins Receptors, LH Spondyloepiphyseal dysplasia, Omani type testosterone dehydrogenase [NAD(P)] activity |
| Content Type | Text |
| Resource Type | Article |
