Loading...
Please wait, while we are loading the content...
Similar Documents
K+ current distribution in rat sub-epicardial ventricular myocytes
| Content Provider | Semantic Scholar |
|---|---|
| Author | Komukai, Kimiaki Brette, Fabien Yamanushi, Tomoko T. Orchard, Clive H. |
| Copyright Year | 2002 |
| Abstract | Abstract. In the rat ventricle, the transient outward K+ current (ITO) is carried by heteromeric channels composed of Kv4.2 and Kv4.3. However its distribution in the cell membrane is unclear: immunohistochemical studies of Kv4.2 distribution in the cardiac ventricular cell membrane have given equivocal results, and there are no corresponding studies of Kv4.3. We therefore used detubulated cardiac cells to investigate the functional distribution of ITO between the t-tubules and surface membrane. ITO, the delayed rectifier (IK), the inward rectifier (IK1) and steady-state (ISS) K+ currents were monitored using the patch-clamp technique in control and formamide-treated (detubulated) cells from rat left ventricular sub-epicardium. Formamide treatment decreased cell capacitance by 20%, did not significantly change the density of ITO, IK or IK1 but decreased the density of ISS and L-type Ca current (ICa). These data suggest that ITO, IK, and IK1 are uniformly distributed between the surface and t-tubule membranes, but that ISS and ICa are concentrated in the t-tubules. |
| Starting Page | 532 |
| Ending Page | 538 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/s00424-002-0851-8 |
| Alternate Webpage(s) | https://page-one.springer.com/pdf/preview/10.1007/s00424-002-0851-8 |
| PubMed reference number | 12136273 |
| Alternate Webpage(s) | https://doi.org/10.1007/s00424-002-0851-8 |
| Journal | Medline |
| Volume Number | 444 |
| Journal | Pflügers Archiv |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
