NDLI: Molecular characterization and heterologous expression of quinate dehydrogenase gene from Gluconobacter oxydans IFO3244

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Molecular characterization and heterologous expression of quinate dehydrogenase gene from Gluconobacter oxydans IFO3244

Content Provider	Semantic Scholar
Author	Vangnai, Alisa S. Promden, Worrawat De-Eknamkul, Wanchai Matsushita, Kazunobu Toyama, Hirohide
Copyright Year	2010
Abstract	The quinate dehydrogenase (QDH) from Gluconobacter oxydans IFO3244 exhibits high affinity for quinate, suggesting its application in shikimate production. Nucleotide sequence analysis of the qdh gene revealed a full-length of 2475-bp encoding an 824-amino acid protein. The qdh gene has the unusual TTG translation initiation codon. Conserved regions and a signature sequence for the quinoprotein family were observed. Phylogenetic analysis demonstrated relatedness of QDH from G. oxydans to other quinate/shikimate dehydrogenases with the highest similarity (56%) with that of Acinetobacter calcoaceticus ADP1 and lower similarity (36%) with a membrane-bound glucose dehydrogenase of Escherichia coli. The function of the gene coding for QDH was confirmed by heterologous gene expression in pyrroloquinoline quinone-synthesizing Pseudomonas putida HK5.
Starting Page	452
Ending Page	459
Page Count	8
File Format	PDF HTM / HTML
DOI	10.1134/S0006297910040085
PubMed reference number	20618134
Journal	Medline
Volume Number	75
Alternate Webpage(s)	http://dspace.bru.ac.th/xmlui/bitstream/handle/123456789/4100/Quinate-Dehydrogenase-from-Gluconobacter-oxydans-IFO3244.pdf?isAllowed=y&sequence=1
Alternate Webpage(s)	https://doi.org/10.1134/S0006297910040085
Journal	Biochemistry (Moscow)
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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